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验证数据展示

  • Dot Blot experiment of / using 86890-2-RR

    Dot Blot experiment of / using 86890-2-RR

    Dot blot analysis was used to confirm the specificity of 86890-2-RR Di-Methyl-Histone H3 (Lys27) antibody. peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (μg/mL) spotted is indicated next to each row.

  • WB analysis using 86890-2-RR

    WB analysis using 86890-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86890-2-RR (Di-Methyl-Histone H3 (Lys27) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis using 86890-2-RR

    WB analysis using 86890-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86890-2-RR (Di-Methyl-Histone H3 (Lys27) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 86890-2-RR

    IF Staining of HeLa using 86890-2-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Di-Methyl-Histone H3 (Lys27) antibody (86890-2-RR, Clone: 251846E11 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

Di-Methyl-Histone H3 (Lys27) Recombinant monoclonal antibody

Di-Methyl-Histone H3 (Lys27) Uni-rAb® Recombinant Antibody for WB, IF/ICC, Dot Blot, ELISA
Cat No. 86890-2-RR

产品说明书

CloneNo. 251846E11

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, Dot Blot, ELISA

别名

DiMethylHistone H3, Di-Methyl-Histone H3, Di-MethylHistone H3 (Lys27), Di-MethylHistone H3 Lys27, Di-Methyl-Histone-H3

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, NIH/3T3 cells, HEK-293T cells, K-562 cells, MCF-7 cells, mouse liver tissue, rat liver tissue
Positive IF/ICC detected inHeLa cells
Positive Dot Blot detected in/ /
Planning an IHC experiment? We recommend our IHCeasy Phospho-Histone H3 (Ser10) Ready-To-Use IHC Kit. Phospho-Histone H3 (Ser10) primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
DOT BLOTDOT BLOT : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

86890-2-RR targets Di-Methyl-Histone H3 (Lys27) in WB, IF/ICC, Dot Blot, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, Dot Blot, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 histone cluster 1, H3a
别名 DiMethylHistone H3, Di-Methyl-Histone H3, Di-MethylHistone H3 (Lys27), Di-MethylHistone H3 Lys27, Di-Methyl-Histone-H3
观测分子量17 kDa
GenBank蛋白编号BC066245
基因名称 HIST1H3A
Gene ID (NCBI) 8350
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP68431
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for Di-Methyl-Histone H3 (Lys27) antibody 86890-2-RRDownload protocol
WB protocol for Di-Methyl-Histone H3 (Lys27) antibody 86890-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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