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验证数据展示

  • WB analysis using 86888-1-RR

    WB analysis using 86888-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86888-1-RR (PPP1R10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 86888-1-RR

    IF Staining of HepG2 using 86888-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PPP1R10 antibody (86888-1-RR, Clone: 251508G3 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

PPP1R10 Recombinant monoclonal antibody

PPP1R10 Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 86888-1-RR

产品说明书

CloneNo. 251508G3

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

CAT53, FB19, MHC class I region proline-rich protein CAT53, p99, PNUTS

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

86888-1-RR targets PPP1R10 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag20091

Product name: Recombinant human PPP1R10 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-318 aa of BC150310

Sequence: MGSGPIDPKELLKGLDSFLNRDGEVKSVDGISKIFSLMKEARKMVSRCTYLNILLQTRSPEILVKFIDVGGYKLLNNWLTYSKTTNNIPLLQQILLTLQHLPLTVDHLKQNNTAKLVKQLSKSSEDEELRKLASVLVSDWMAVIRSQSSTQPAEKDKKKRKDEGKSRTTLPERPLTEVKAETRAEEAPEKKREKPKSLRTTAPSHAKFRSTGLELETPSLVPVKKNASTVVVSDKYNLKPIPLKRQSNVAAPGDATPPAEKKYKPLNTTPNATKEIKVKIIPPQPMEGLGFLDALNSAPVPGIKIKKKKKVLSPTAAK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 protein phosphatase 1, regulatory (inhibitor) subunit 10
别名 CAT53, FB19, MHC class I region proline-rich protein CAT53, p99, PNUTS
计算分子量 940 aa, 99 kDa
观测分子量 99-110 kDa
GenBank蛋白编号BC150310
基因名称 PPP1R10
Gene ID (NCBI) 5514
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ96QC0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for PPP1R10 antibody 86888-1-RRDownload protocol
WB protocol for PPP1R10 antibody 86888-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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