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验证数据展示

  • WB analysis using 86846-2-RR (same clone as 86846-2-PBS)

    WB analysis using 86846-2-RR (same clone as 86846-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86846-2-RR (RGS7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86846-2-PBS in a different storage buffer formulation.

  • WB analysis using 86846-2-RR (same clone as 86846-2-PBS)

    WB analysis using 86846-2-RR (same clone as 86846-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86846-2-RR (RGS7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86846-2-PBS in a different storage buffer formulation.

  • IF Staining of U-87 MG using 86846-2-RR (same clone as 86846-2-PBS)

    IF Staining of U-87 MG using 86846-2-RR (same clone as 86846-2-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed U-87 MG cells using RGS7 antibody (86846-2-RR, Clone: 251849E9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86846-2-PBS in a different storage buffer formulation.

  • Cytometric bead array sample test of MP02701-1

    Cytometric bead array sample test of MP02701-1

    The mean RGS7 concentration was determined to be 5.9 ng/mL in SH-SY5Y cell extract based on a 1.2 mg/mL extract load, 237.6 ng/mL in Mouse brain tissue extract based on a 3.2 mg/mL extract load.

  • Cytometric bead array standard curve of MP02701-1

    Cytometric bead array standard curve of MP02701-1

    Cytometric bead array standard curve of MP02701-1, RGS7 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 86846-2-PBS. Detection antibody: 86846-1-PBS. Standard: Ag4982. Range: 0.781-100 ng/mL.

RGS7 Recombinant monoclonal antibody, PBS Only (Capture)

RGS7 Uni-rAb® Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 86846-2-PBS

产品说明书

CloneNo. 251849E9

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, Cytometric bead array, Indirect ELISA

别名

regulator of G protein signaling 7, Regulator of G-protein signaling 7

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

86846-2-PBS targets RGS7 as part of a matched antibody pair:

MP02701-1: 86846-2-PBS capture and 86846-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag4982

Product name: Recombinant human RGS7 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 139-487 aa of BC022009

Sequence: NKARLELADYEAESLARLQRAFARKWEFIFMQAEAQAKVDKKRDKIERKILDSQERAFWDVHRPVPGCVNTTEVDIKKSSRMRNPHKTRKSVYGLQNDIRSHSPTHTPTPETKPPTEDELQQQIKYWQIQLDRHRLKMSKVADSLLSYTEQYLEYDPFLLPPDPSNPWLSDDTTFWELEASKEPSQQRVKRWGFGMDEALKDPVGREQFLKFLESEFSSENLRFWLAVEDLKKRPIKEVPSRVQEIWQEFLAPGAPSAINLDSKSYDKTTHNVKEPGRYTFEDAQEHIYKLMKSDSYPRFIRSSAYQELLQAKKKSGNSMDRRTSFEKFAQNVGKSLTSKRLTSLAQSY

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 regulator of G-protein signaling 7
别名 regulator of G protein signaling 7, Regulator of G-protein signaling 7
计算分子量 58 kDa
观测分子量 58 kDa
GenBank蛋白编号BC022009
基因名称 RGS7
Gene ID (NCBI) 6000
RRIDAB_3745158
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP49802
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Regulator of G-protein signaling 7 (RGS7) expression is both necessary and sufficient to promote doxorubicin-dependent acetylation and activation of NF-κB subunit p65 and resultant production of pro-inflammatory cytokines from cardiomyocytes (PMID: 37589751). RGS7 promotes apoptosis in cardiac myocytes by increasing oxidative stress and impairing mitochondrial function, actions that involve a direct and functional interaction between RGS7 and the Ca2+/calmodulin-dependent kinase CaMKII (PMID: 36574707). In non-neuronal cell types, it is possible that a greater proportion of RGS7 may be present in the cytosol where it is free to interact with additional effectors (PMID: 36574707).

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