验证数据展示
经过测试的应用
| Positive WB detected in | SH-SY5Y cells, mouse brain tissue, rat brain tissue, U-87 MG cells, SK-N-SH cells, Neuro-2a cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
86846-2-RR targets RGS7 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag4982 Product name: Recombinant human RGS7 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 139-487 aa of BC022009 Sequence: NKARLELADYEAESLARLQRAFARKWEFIFMQAEAQAKVDKKRDKIERKILDSQERAFWDVHRPVPGCVNTTEVDIKKSSRMRNPHKTRKSVYGLQNDIRSHSPTHTPTPETKPPTEDELQQQIKYWQIQLDRHRLKMSKVADSLLSYTEQYLEYDPFLLPPDPSNPWLSDDTTFWELEASKEPSQQRVKRWGFGMDEALKDPVGREQFLKFLESEFSSENLRFWLAVEDLKKRPIKEVPSRVQEIWQEFLAPGAPSAINLDSKSYDKTTHNVKEPGRYTFEDAQEHIYKLMKSDSYPRFIRSSAYQELLQAKKKSGNSMDRRTSFEKFAQNVGKSLTSKRLTSLAQSY 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | regulator of G-protein signaling 7 |
| 别名 | regulator of G protein signaling 7, Regulator of G-protein signaling 7 |
| 计算分子量 | 58 kDa |
| 观测分子量 | 58 kDa |
| GenBank蛋白编号 | BC022009 |
| 基因名称 | RGS7 |
| Gene ID (NCBI) | 6000 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P49802 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Regulator of G-protein signaling 7 (RGS7) expression is both necessary and sufficient to promote doxorubicin-dependent acetylation and activation of NF-κB subunit p65 and resultant production of pro-inflammatory cytokines from cardiomyocytes (PMID: 37589751). RGS7 promotes apoptosis in cardiac myocytes by increasing oxidative stress and impairing mitochondrial function, actions that involve a direct and functional interaction between RGS7 and the Ca2+/calmodulin-dependent kinase CaMKII (PMID: 36574707). In non-neuronal cell types, it is possible that a greater proportion of RGS7 may be present in the cytosol where it is free to interact with additional effectors (PMID: 36574707).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RGS7 antibody 86846-2-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
