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验证数据展示

  • WB analysis using 86808-1-RR

    WB analysis using 86808-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86808-1-RR (TRAIP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 86808-1-RR

    IHC staining of human breast cancer using 86808-1-RR

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 86808-1-RR (TRAIP antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TRAIP antibody included
  • IHC staining of human breast cancer using 86808-1-RR

    IHC staining of human breast cancer using 86808-1-RR

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 86808-1-RR (TRAIP antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TRAIP antibody included
  • Affinity and Kinetic Characterization of 86808-1-RR

    Affinity and Kinetic Characterization of 86808-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86808-1-RR against Human TRAIP were performed. The affinity constant is below 1 pM.

TRAIP Recombinant monoclonal antibody

TRAIP Uni-rAb® Recombinant Antibody for WB, IHC, ELISA
Cat No. 86808-1-RR

产品说明书

CloneNo. 251370C8

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

TRIP, EC:2.3.2.27, RING finger protein 206, RNF206, TRAF interacting protein

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, A549 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy TRAIP Ready-To-Use IHC Kit. TRAIP primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

86808-1-RR targets TRAIP in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag0491

Product name: Recombinant human TRAIP protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-204 aa of BC000310

Sequence: MPIRALCTICSDFFDHSRDVAAIHCGHTFHLQCLIQWFETAPSRTCPQCRIQVGKRTIINKLFFDLAQEEENVLDAEFLKNELDNVRAQLSQKDKEKRDSQVIIDTLRDTLEERNATVVSLQQALGKAEMLCSTLKKQMKYLEQQQDETKQAQEEARRLRSKMKTMEQIELLLQSQRPEVEEMIRDMGVGQSAVEQLAVYCVSL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 TRAF interacting protein
别名 TRIP, EC:2.3.2.27, RING finger protein 206, RNF206, TRAF interacting protein
计算分子量 53 kDa
观测分子量 53 kDa
GenBank蛋白编号BC000310
基因名称 TRAIP
Gene ID (NCBI) 10293
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9BWF2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

E3 ubiquitin-protein ligase TRAIP (TRAIP) is also named RING finger protein 206 (RNF206) and TRAF-interacting protein (TRIP). TRAIP, an E3 ligase containing a RING domain, has emerged as a significant contributor to maintaining genome integrity and TRAIP inhibits proliferation and migration of BLCA cells (PMID: 38123820). TRAIP helps replisomes overcome DNA interstrand crosslinks and DNA-protein crosslinks, whereas in mitosis it triggers disassembly of all replisomes that remain on chromatin (PMID: 33317933, PMID: 30842657).

实验方案

Product Specific Protocols
IHC protocol for TRAIP antibody 86808-1-RRDownload protocol
WB protocol for TRAIP antibody 86808-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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