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验证数据展示

  • WB analysis using 86775-3-RR

    WB analysis using 86775-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86775-3-RR (HFE2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 86775-3-RR

    IF Staining of HepG2 using 86775-3-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using HFE2 antibody (86775-3-RR, Clone: 251477G9 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 86775-3-RR

    Affinity and Kinetic Characterization of 86775-3-RR

    Biolayer interferometry (BLl) kinetic assays of 86775-3-RR against Human HFE2 were performed. The affinity constant is below 1 pM.

HFE2/Hemojuvelin Recombinant monoclonal antibody

HFE2/Hemojuvelin Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 86775-3-RR

产品说明书

CloneNo. 251477G9

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, ELISA

别名

Hemojuvelin, HFE2, Hemochromatosis type 2 protein, Hemojuvelin BMP coreceptor, HFE2A

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293T cells, HeLa cells, HepG2 cells, human platelets, human skeletal muscle tissue, human heart tissue, mouse liver tissue
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

86775-3-RR targets HFE2/Hemojuvelin in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag2354

Product name: Recombinant human HFE2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 227-426 aa of BC017926

Sequence: MQECIDQKVYQAEVDNLPVAFEDGSINGGDRPGGSSLSIQTANPGNHVEIQAAYIGTTIIIRQTAGQLSFSIKVAEDVAMAFSAEQDLQLCVGGCPPSQRLSRSERNRRGAITIDTARRLCKEGLPVEDAYFHSCVFDVLISGDPNFTVAAQAALEDARAFLPDLEKLHLFPSDAGVPLSSATLLAPLLSGLFVLWLCIQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 hemochromatosis type 2 (juvenile)
别名 Hemojuvelin, HFE2, Hemochromatosis type 2 protein, Hemojuvelin BMP coreceptor, HFE2A
计算分子量 426 aa, 45 kDa
观测分子量45 kDa
GenBank蛋白编号BC017926
基因名称 HFE2
Gene ID (NCBI) 148738
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ6ZVN8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HFE2, also known as Hemojuvelin (HJV), is a GPI-anchored protein that binds to new proteins and bone morphogenetic proteins (BMP2 and BMP4) and can be released from the cell membrane. HFE2 plays a key role in the regulation of hepcidin, which regulates the absorption and recycling of iron.(PMID: 17331953; PMID: 17938254)

实验方案

Product Specific Protocols
IF protocol for HFE2/Hemojuvelin antibody 86775-3-RRDownload protocol
WB protocol for HFE2/Hemojuvelin antibody 86775-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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