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验证数据展示

  • WB analysis using 86512-3-RR (same clone as 86512-3-PBS)

    WB analysis using 86512-3-RR (same clone as 86512-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86512-3-RR (CD73 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86512-3-PBS in a different storage buffer formulation.

  • Sandwich ELISA sample test of MP02481-2

    Sandwich ELISA sample test of MP02481-2

    The mean CD73 concentration was determined to be 39.19 ng/mL in U-87 MG cell extract based on a 1.50 mg/mL extract load and 31.20 ng/mL in HT-29 cell extract based on a 1.10 mg/mL extract load.

  • Sandwich ELISA sample test of MP02481-2

    Sandwich ELISA sample test of MP02481-2

    Plasma of six individual healthy human donors was measured. The CD73 concentration of detected samples was determined to be 17.66 ng/mL with a range of 9.53-35.23 ng/mL.

  • Sandwich ELISA standard curve of MP02481-2

    Sandwich ELISA standard curve of MP02481-2

    Sandwich ELISA standard curve of MP02481-2, Human CD73 Recombinant Matched Antibody Pair - PBS only. 86512-5-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg2974. 86512-3-PBS was HRP conjugated as the detection antibody. Range: 0.313-20 ng/mL.

  • IF Staining of U-251 using 86512-3-RR (same clone as 86512-3-PBS)

    IF Staining of U-251 using 86512-3-RR (same clone as 86512-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using CD73 antibody (86512-3-RR, Clone: 251250H3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86512-3-PBS in a different storage buffer formulation.

CD73 Recombinant monoclonal antibody, PBS Only (Detector)

CD73 Uni-rAb® Recombinant Antibody for WB, IF/ICC, Sandwich ELISA, Indirect ELISA
Cat No. 86512-3-PBS

产品说明书

CloneNo. 251250H3

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, Sandwich ELISA, Indirect ELISA

别名

NT5E, 5 NT, 5 nucleotidase, 5' nucleotidase, ecto (CD73), 5'-deoxynucleotidase

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

86512-3-PBS targets CD73 as part of a matched antibody pair:

MP02481-2: 86512-5-PBS capture and 86512-3-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, Sandwich ELISA, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Eg2974

Product name: Recombinant Human CD73 protein (rFc Tag)

Source: mammalian cells-derived, V37

Tag: C-rFc

Domain: 27-547 aa of NM_002526.3

Sequence: WELTILHTNDVHSRLEQTSEDSSKCVNASRCMGGVARLFTKVQQIRRAEPNVLLLDAGDQYQGTIWFTVYKGAEVAHFMNALRYDAMALGNHEFDNGVEGLIEPLLKEAKFPILSANIKAKGPLASQISGLYLPYKVLPVGDEVVGIVGYTSKETPFLSNPGTNLVFEDEITALQPEVDKLKTLNVNKIIALGHSGFEMDKLIAQKVRGVDVVVGGHSNTFLYTGNPPSKEVPAGKYPFIVTSDDGRKVPVVQAYAFGKYLGYLKIEFDERGNVISSHGNPILLNSSIPEDPSIKADINKWRIKLDNYSTQELGKTIVYLDGSSQSCRFRECNMGNLICDAMINNNLRHTDEMFWNHVSMCILNGGGIRSPIDERNNGTITWENLAAVLPFGGTFDLVQLKGSTLKKAFEHSVHRYGQSTGEFLQVGGIHVVYDLSRKPGDRVVKLDVLCTKCRVPSYDPLKMDEVYKVILPNFLANGGDGFQMIKDELLRHDSGDQDINVVSTYISKMKVIYPAVEGRIK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 5'-nucleotidase, ecto (CD73)
别名 NT5E, 5 NT, 5 nucleotidase, 5' nucleotidase, ecto (CD73), 5'-deoxynucleotidase
计算分子量63 kDa
观测分子量 63-70 kDa
GenBank蛋白编号NM_002526.3
基因名称 CD73
Gene ID (NCBI) 4907
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP21589-1
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NT5E(5'-nucleotidase) is also named as NT5, NTE,CD73,ecto-5'-nucleotidase, and belongs to the 5'-nucleotidase family. It catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP.The enzyme occurs mainly as a dimer and the wide distribution of NT5E in the rat hippocampus, suggesting their involvement in the control of the purinergic signaling(PMID:17619139). This protein has two isoforms with the molecular weight of 63 kDa and 58 kDa and four glycosylation sites with a signalpeptide. It can exsit as a dimer(PMID:17619139). It can be detected the band of 55 kDa, 64 kDa, 70 kDa, 110 kDa by western blot(PMID:17619139; 12370277; 21533188). Defects in NT5E are the cause of calcification of joints and arteries (CALJA).

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