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验证数据展示

  • WB analysis using 86473-3-RR

    WB analysis using 86473-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86473-3-RR (BAF170 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 86473-3-RR

    Affinity and Kinetic Characterization of 86473-3-RR

    Biolayer interferometry (BLl) kinetic assays of 86473-3-RR against Human BAF170 were performed. The affinity constant is 4.64 nM.

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BAF170 Recombinant monoclonal antibody

BAF170 Uni-rAb® Recombinant Antibody for WB, ELISA
Cat No. 86473-3-RR

产品说明书

CloneNo. 251330D11

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, ELISA

别名

SMARCC2, BRG1-associated factor 170, CRACC2, Rsc8, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily C member 2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inLNCaP cells, A431 cells, HeLa cells, RAW 264.7 cells, C6 cells, NIH/3T3 cells, PC-12 cells
Planning an IHC experiment? We recommend our IHCeasy SMARCC2 Ready-To-Use IHC Kit. SMARCC2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

86473-3-RR targets BAF170 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag2634

Product name: Recombinant human BAF170 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 300-647 aa of BC013045

Sequence: KRSPSPSPTPEAKKKNAKKGPSTPYTKSKRGHREEEQEDLTKDMDEPSPVPNVEEVTLPKTVNTKKDSESAPVKGGTMTDLDEQEDESMETTGKDEDENSTGNKGEQTKNPDLHEDNVTEQTHHIIIPSYAAWFDYNSVHAIERRALPEFFNGKNKSKTPEIYLAYRNFMIDTYRLNPQEYLTSTACRRNLAGDVCAIMRVHAFLEQWGLINYQVDAESRPTPMGPPPTSHFHVLADTPSGLVPLQPKTPQGRQVDADTKAGRKGKELDDLVPETAKGKPELQTSASQQMLNFPDKGKEKPTDMQNFGLRTDMYTKKNVPSKSKAAASATREWTEQETLLLLEALEMY

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily c, member 2
别名 SMARCC2, BRG1-associated factor 170, CRACC2, Rsc8, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily C member 2
计算分子量 1214 aa, 132 kDa
观测分子量170 kDa
GenBank蛋白编号BC013045
基因名称 BAF170
Gene ID (NCBI) 6601
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ8TAQ2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BAF170, also named as SWI/SNF complex 170 kDa subunit or SMARCC2, is a 1214 amino acid protein, which belongs to the SMARCC family. BAF170 is ubiquitously expressed. BAF170 is involved in transcriptional activation and repression of select genes by chromatin remodeling. BAF170 may be required for CoREST dependent repression of neuronal specific gene promoters in non-neuronal cells. BAF170 belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). The calcualted molecular weight of BAF170, is 133 kDa, but modified BAF170 is about 170 kDa. (PMID: 8804307)

实验方案

Product Specific Protocols
WB protocol for BAF170 antibody 86473-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols