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验证数据展示

  • WB analysis using 86340-2-RR (same clone as 86340-2-PBS)

    WB analysis using 86340-2-RR (same clone as 86340-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86340-2-RR (Alpha Actinin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86340-2-PBS in a different storage buffer formulation.

  • Sample test of MP02360-1

    Sample test of MP02360-1

    The mean Alpha Actinin concentration was determined to be 254.6 ng/mL in NIH/3T3 cell extract based on a 1.4 mg/mL extract load.

  • IHC staining of mouse heart using 86340-2-RR (same clone as 86340-2-PBS)

    IHC staining of mouse heart using 86340-2-RR (same clone as 86340-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 86340-2-RR (Alpha Actinin antibody) at dilution of 1:1500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86340-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Alpha Actinin antibody included
  • IHC staining of mouse heart using 86340-2-RR (same clone as 86340-2-PBS)

    IHC staining of mouse heart using 86340-2-RR (same clone as 86340-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 86340-2-RR (Alpha Actinin antibody) at dilution of 1:1500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86340-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Alpha Actinin antibody included
  • IF Staining of H9C2 using 86340-2-RR (same clone as 86340-2-PBS)

    IF Staining of H9C2 using 86340-2-RR (same clone as 86340-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed H9C2 cells using Alpha Actinin antibody (86340-2-RR, Clone: 250833F4 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86340-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP02360-1

    Cytometric bead array standard curve of MP02360-1

    Cytometric bead array standard curve of MP02360-1, Alpha Actinin Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 86340-2-PBS. Detection antibody: 86340-1-PBS. Standard: Ag1859. Range: 0.781-100 ng/mL.

Alpha Actinin Recombinant antibody, PBS Only (Capture)

Alpha Actinin Recombinant Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA, Sample test
Cat No. 86340-2-PBS

产品说明书

CloneNo. 250833F4

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA, Sample test

别名

ACTN1, Alpha actinin 1, actin, actinin, alpha 1, ACTN 1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy Alpha Actinin Ready-To-Use IHC Kit. Alpha Actinin primary antibody included.

产品信息

86340-2-PBS targets Alpha Actinin as part of a matched antibody pair:

MP02360-1: 86340-2-PBS capture and 86340-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA, Sample test Application Description
经测试反应性 human, mouse, rat
免疫原 Alpha Actinin fusion protein Ag1859 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 actinin, alpha 1
别名 ACTN1, Alpha actinin 1, actin, actinin, alpha 1, ACTN 1
计算分子量 103 kDa
观测分子量 100-105 kDa
GenBank蛋白编号BC015766
基因名称 Alpha Actinin
Gene ID (NCBI) 87
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP12814
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Alpha Actinins are highly conserved actin-binding proteins that form antiparallel homodimers with the actin-binding domains on each end of the molecule, allowing crosslinking of actin molecules. There are four alpha actinin isoforms identified: two ubiquitously expressed cytoskeletal isoforms (ACTN1 and ACTN4) and two striated muscle-specific sarcomeric isoforms (ACTN2 and ACTN3). ACTN1 is also alternatively spliced to form smooth muscle or brain specific isoforms. This antibody recognize endogenous ACTN1, while it may cross-react with ACTN2 and ACTN4.