Alpha Actinin Recombinant antibody

Alpha Actinin Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 86340-2-RR

产品说明书

CloneNo. 250833F4

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, ELISA

ACTN1, Alpha actinin 1, actin, actinin, alpha 1, ACTN 1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存


经过测试的应用

Positive WB detected inHeLa cells, PANC-1 cells, NIH/3T3 cells, C6 cells, mouse spleen tissue, rat heart tissue
Planning an IHC experiment? We recommend our IHCeasy Alpha Actinin Ready-To-Use IHC Kit. Alpha Actinin primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

86340-2-RR targets Alpha Actinin in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Alpha Actinin fusion protein Ag1859 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 actinin, alpha 1
别名 ACTN1, Alpha actinin 1, actin, actinin, alpha 1, ACTN 1
计算分子量 103 kDa
观测分子量 100-105 kDa
GenBank蛋白编号BC015766
基因名称 Alpha Actinin
Gene ID (NCBI) 87
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP12814
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Alpha Actinins are highly conserved actin-binding proteins that form antiparallel homodimers with the actin-binding domains on each end of the molecule, allowing crosslinking of actin molecules. There are four alpha actinin isoforms identified: two ubiquitously expressed cytoskeletal isoforms (ACTN1 and ACTN4) and two striated muscle-specific sarcomeric isoforms (ACTN2 and ACTN3). ACTN1 is also alternatively spliced to form smooth muscle or brain specific isoforms. This antibody recognize endogenous ACTN1, while it may cross-react with ACTN2 and ACTN4.

实验方案

Product Specific Protocols
WB protocol for Alpha Actinin antibody 86340-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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