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验证数据展示

  • WB analysis using 86297-1-RR

    WB analysis using 86297-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86297-1-RR (ALDH4A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 86297-1-RR

    Affinity and Kinetic Characterization of 86297-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86297-1-RR against Human ALDH4A1 were performed. The affinity constant is below 1 pM.

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ALDH4A1 Recombinant monoclonal antibody

ALDH4A1 Uni-rAb® Recombinant Antibody for WB, ELISA
Cat No. 86297-1-RR

产品说明书

CloneNo. 250957F8

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, ELISA

别名

ALDH4, ALDH4A 1, Delta-1-pyrroline-5-carboxylate dehydrogenase, mitochondrial, EC:1.2.1.88, L-glutamate gamma-semialdehyde dehydrogenase

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHepG2 cells, K-562 cells, A549 cells, BT-474 cells, mouse skeletal muscle tissue, rat skeletal muscle tissue, human skeletal muscle tissue
Planning an IHC experiment? We recommend our IHCeasy ALDH4A1 Ready-To-Use IHC Kit. ALDH4A1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

86297-1-RR targets ALDH4A1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag2147

Product name: Recombinant human ALDH4A1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 246-563 aa of BC023600

Sequence: YRILREAGLPPNIIQFVPADGPLFGDTVTSSEHLCGINFTGSVPTFKHLWKQVAQNLDRFHTFPRLAGECGGKNFHFVHRSADVESVVSGTLRSAFEYGGQKCSACSRLYVPHSLWPQIKGRLLEEHSRIKVGDPAEDFGTFFSAVIDAKSFARIKKWLEHARSSPSLTILAGGKCDDSVGYFVEPCIVESKDPQEPIMKEEIFGPVLSVYVYPDDKYKETLQLVDSTTSYGLTGAVFSQDKDVVQEATKVLRNAAGNFYINDKSTGSIVGQQPFGGARASGTNDKPGGPHYILRWTSPQVIKETHKPLGDWSYAYMQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 aldehyde dehydrogenase 4 family, member A1
别名 ALDH4, ALDH4A 1, Delta-1-pyrroline-5-carboxylate dehydrogenase, mitochondrial, EC:1.2.1.88, L-glutamate gamma-semialdehyde dehydrogenase
计算分子量 563 aa, 62 kDa
观测分子量 62 kDa
GenBank蛋白编号BC023600
基因名称 ALDH4A1
Gene ID (NCBI) 8659
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP30038
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ALDH4A1(Aldehyde dehydrogenase family 4 member A1) is also named as ALDH4, P5CDH,P5C dehydrogenase and belongs to the aldehyde dehydrogenase family. P5CDH is a mitochondrial matrix NAD(+)-dependent dehydrogenase which catalyzes the second step of the proline degradation pathway, converting pyrroline-5-carboxylate to glutamate. Defects in ALDH4A1 are the cause of hyperprolinemia type 2 (HP-2).

实验方案

Product Specific Protocols
WB protocol for ALDH4A1 antibody 86297-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols