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验证数据展示

  • WB analysis using 86217-1-RR (same clone as 86217-1-PBS)

    WB analysis using 86217-1-RR (same clone as 86217-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86217-1-RR (PRPS1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86217-1-PBS in a different storage buffer formulation.

  • IF Staining of HEK-293 using 86217-1-RR (same clone as 86217-1-PBS)

    IF Staining of HEK-293 using 86217-1-RR (same clone as 86217-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HEK-293 cells using PRPS1 antibody (86217-1-RR, Clone: 250201C1 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86217-1-PBS in a different storage buffer formulation.

  • Cytometric bead array sample test of MP02295-1

    Cytometric bead array sample test of MP02295-1

    The mean PRPS1 concentration was determined to be 62.7 ng/mL in Jurkat cell extract based on a 1.2 mg/mL extract load.

  • Cytometric bead array standard curve of MP02295-1

    Cytometric bead array standard curve of MP02295-1

    Cytometric bead array standard curve of MP02295-1, PRPS1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 86217-2-PBS. Detection antibody: 86217-1-PBS. Standard: Ag7907. Range: 1.56-200 ng/mL.

  • Affinity and Kinetic Characterization of 86217-1-RR

    Affinity and Kinetic Characterization of 86217-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86217-1-RR against Human PRPS1 were performed. The affinity constant is 2.03 nM.

PRPS1 Recombinant monoclonal antibody, PBS Only (Detector)

PRPS1 Uni-rAb® Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 86217-1-PBS

产品说明书

CloneNo. 250201C1

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, Cytometric bead array, Indirect ELISA

别名

ARTS, CMTX5, EC:2.7.6.1, KIAA0967, PPRibP

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

86217-1-PBS targets PRPS1 as part of a matched antibody pair:

MP02295-1: 86217-2-PBS capture and 86217-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag7907

Product name: Recombinant human PRPS1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-318 aa of BC001605

Sequence: MPNIKIFSGSSHQDLSQKIADRLGLELGKVVTKKFSNQETCVEIGESVRGEDVYIVQSGCGEINDNLMELLIMINACKIASASRVTAVIPCFPYARQDKKDKSRAPISAKLVANMLSVAGADHIITMDLHASQIQGFFDIPVDNLYAEPAVLKWIRENISEWRNCTIVSPDAGGAKRVTSIADRLNVDFALIHKERKKANEVDRMVLVGDVKDRVAILVDDMADTCGTICHAADKLLSAGATRVYAILTHGIFSGPAISRINNACFEAVVVTNTIPQEDKMKHCSKIQVIDISMILAEAIRRTHNGESVSYLFSHVPL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 phosphoribosyl pyrophosphate synthetase 1
别名 ARTS, CMTX5, EC:2.7.6.1, KIAA0967, PPRibP
计算分子量 318 aa, 35 kDa
观测分子量 33 kDa
GenBank蛋白编号BC001605
基因名称 PRPS1
Gene ID (NCBI) 5631
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP60891
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PRPS1, also named as Ribose-phosphate pyrophosphokinase 1, is a 318 amino acid protein, which belongs to the ribose-phosphate pyrophosphokinase family. PRPS1 can form Homodimer and the active form is probably a hexamer composed of 3 homodimers. PRPS1 catalyzes the synthesis of phosphoribosylpyrophosphate (PRPP) which is essential for nucleotide synthesis. PRPS1 as a module has an association with the Hepatocellular carcinoma.

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