验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, U-87 MG cells, mouse kidney tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
86217-1-RR targets PRPS1 in WB, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 | PRPS1 fusion protein Ag7907 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | phosphoribosyl pyrophosphate synthetase 1 |
| 别名 | ARTS, CMTX5, EC:2.7.6.1, KIAA0967, PPRibP |
| 计算分子量 | 318 aa, 35 kDa |
| 观测分子量 | 33 kDa |
| GenBank蛋白编号 | BC001605 |
| 基因名称 | PRPS1 |
| Gene ID (NCBI) | 5631 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P60891 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PRPS1, also named as Ribose-phosphate pyrophosphokinase 1, is a 318 amino acid protein, which belongs to the ribose-phosphate pyrophosphokinase family. PRPS1 can form Homodimer and the active form is probably a hexamer composed of 3 homodimers. PRPS1 catalyzes the synthesis of phosphoribosylpyrophosphate (PRPP) which is essential for nucleotide synthesis. PRPS1 as a module has an association with the Hepatocellular carcinoma.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PRPS1 antibody 86217-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |