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验证数据展示

  • WB analysis using 86207-1-RR (same clone as 86207-1-PBS)

    WB analysis using 86207-1-RR (same clone as 86207-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86207-1-RR (DKK1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86207-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP02491-1

    Sandwich ELISA standard curve of MP02491-1

    Sandwich ELISA standard curve of MP02491-1, Mouse DKK1 Recombinant Matched Antibody Pair - PBS only. 86207-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg4649. 86207-2-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL

  • Sample test of MP02491-1

    Sample test of MP02491-1

    Serum of eight mice was measured. The mouse DKK1 concentration of detected samples was determined to be 4.72 ng/mL with a range of 2.40 - 7.06 ng/mL.

Mouse DKK1 Recombinant antibody, PBS Only (Capture)

DKK1 Recombinant Antibody for WB, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 86207-1-PBS

产品说明书

CloneNo. 250658B2

宿主/亚型

Rabbit / IgG

种属反应性

mouse, rat

应用

WB, Sandwich ELISA, Indirect ELISA, Sample test

别名

Dickkopf-1, Dickkopf-related protein 1, DKK 1, Dkk-1, hDkk 1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

86207-1-PBS targets DKK1 as part of a matched antibody pair:

MP02491-1: 86207-1-PBS capture and 86207-2-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 mouse, rat
免疫原 DKK1 fusion protein Eg4649 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 dickkopf homolog 1 (Xenopus laevis)
别名 Dickkopf-1, Dickkopf-related protein 1, DKK 1, Dkk-1, hDkk 1
计算分子量29kDa
观测分子量30-35 kDa
GenBank蛋白编号NM_010051.3
基因名称 Dkk1
Gene ID (NCBI) 13380
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO54908
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DKK1, also named a SK and Dickkopf-1, belongs to the dickkopf family. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease. SDS-PAGE and Western blot analysis demonstrated that DKK1 is expressed as a 35-kDa doublet protein, which is larger than the deduced molecular mass of 26 kDa. Sometime DKK1 is expressed as a 42- to 50-kDa secreted protein, with little change observed after glycanase treatment.