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验证数据展示

  • WB analysis of mouse brain using 86097-1-RR (same clone as 86097-1-PBS)

    WB analysis of mouse brain using 86097-1-RR (same clone as 86097-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 86097-1-RR (MAG antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86097-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA sample test of MP02467-1

    Sandwich ELISA sample test of MP02467-1

    The mean MAG concentration was determined to be 1.2 ng/mL in SH-SY5Y cell extract based on a 3.2 mg/mL extract load.

  • Sandwich ELISA standard curve of MP02467-1

    Sandwich ELISA standard curve of MP02467-1

    Sandwich ELISA standard curve of MP02467-1, Human MAG Recombinant Matched Antibody Pair - PBS only. 86097-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg2975. 86097-1-PBS was HRP conjugated as the detection antibody. Range: 46.9-3000 pg/mL.

  • IHC staining of mouse brain using 86097-1-RR (same clone as 86097-1-PBS)

    IHC staining of mouse brain using 86097-1-RR (same clone as 86097-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 86097-1-RR (MAG antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86097-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 86097-1-RR (same clone as 86097-1-PBS)

    IHC staining of mouse brain using 86097-1-RR (same clone as 86097-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 86097-1-RR (MAG antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86097-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse brain using 86097-1-RR (same clone as 86097-1-PBS)

    IF Staining of mouse brain using 86097-1-RR (same clone as 86097-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using MAG antibody (86097-1-RR, Clone: 250555A7 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86097-1-PBS in a different storage buffer formulation.

MAG Recombinant monoclonal antibody, PBS Only (Detector)

MAG Uni-rAb® Recombinant Antibody for WB, IHC, IF-P, Sandwich ELISA, Indirect ELISA
Cat No. 86097-1-PBS

产品说明书

CloneNo. 250555A7

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF-P, Sandwich ELISA, Indirect ELISA

别名

GMA, myelin associated glycoprotein, S MAG, SIGLEC 4A, SIGLEC4A

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

86097-1-PBS targets MAG as part of a matched antibody pair:

MP02467-1: 86097-2-PBS capture and 86097-1-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF-P, Sandwich ELISA, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Eg2975

Product name: Recombinant Human MAG protein (rFc Tag)

Source: mammalian cells-derived, V37

Tag: C-rFc

Domain: 20-516 aa of NM_002361.3

Sequence: GHWGAWMPSSISAFEGTCVSIPCRFDFPDELRPAVVHGVWYFNSPYPKNYPPVVFKSRTQVVHESFQGRSRLLGDLGLRNCTLLLSNVSPELGGKYYFRGDLGGYNQYTFSEHSVLDIVNTPNIVVPPEVVAGTEVEVSCMVPDNCPELRPELSWLGHEGLGEPAVLGRLREDEGTWVQVSLLHFVPTREANGHRLGCQASFPNTTLQFEGYASMDVKYPPVIVEMNSSVEAIEGSHVSLLCGADSNPPPLLTWMRDGTVLREAVAESLLLELEEVTPAEDGVYACLAENAYGQDNRTVGLSVMYAPWKPTVNGTMVAVEGETVSILCSTQSNPDPILTIFKEKQILSTVIYESELQLELPAVSPEDDGEYWCVAENQYGQRATAFNLSVEFAPVLLLESHCAAARDTVQCLCVVKSNPEPSVAFELPSRNVTVNESEREFVYSERSGLVLTSILTLRGQAQAPPRVICTARNLYGAKSLELPFQGAHRLMWAKIGP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 myelin associated glycoprotein
别名 GMA, myelin associated glycoprotein, S MAG, SIGLEC 4A, SIGLEC4A
计算分子量69 kDa
观测分子量 100 kDa
GenBank蛋白编号NM_002361.3
基因名称 MAG
Gene ID (NCBI) 4099
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP20916-1
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MAG (myelin associated glycoprotein) is a 100-kDa transmembrane glycoprotein that is a member of the SIGLEC family of proteins. MAG is localized in periaxonal Schwann cell and oligodendroglial membranes of myelin sheaths where it functions in glia-axon interactions (PMID: 17241126). It is a functional ligand for the Nogo-66 receptor (PMID: 12089450). MAG is a cell adhesion molecule for postnatal neural development and is thought to be involved in the process of myelination.

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