• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis of mouse brain using 86097-1-RR

    WB analysis of mouse brain using 86097-1-RR

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 86097-1-RR (MAG antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 86097-1-RR

    IHC staining of mouse brain using 86097-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 86097-1-RR (MAG antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 86097-1-RR

    IHC staining of mouse brain using 86097-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 86097-1-RR (MAG antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

MAG Recombinant antibody

MAG Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA
Cat No. 86097-1-RR

产品说明书

CloneNo. 250555A7

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

GMA, myelin associated glycoprotein, S MAG, SIGLEC 4A, SIGLEC4A

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inmouse brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

86097-1-RR targets MAG in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 MAG fusion protein Eg2975 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 myelin associated glycoprotein
别名 GMA, myelin associated glycoprotein, S MAG, SIGLEC 4A, SIGLEC4A
计算分子量69 kDa
观测分子量 100 kDa
GenBank蛋白编号NM_002361.3
基因名称 MAG
Gene ID (NCBI) 4099
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP20916-1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MAG (myelin associated glycoprotein) is a 100-kDa transmembrane glycoprotein that is a member of the SIGLEC family of proteins. MAG is localized in periaxonal Schwann cell and oligodendroglial membranes of myelin sheaths where it functions in glia-axon interactions (PMID: 17241126). It is a functional ligand for the Nogo-66 receptor (PMID: 12089450). MAG is a cell adhesion molecule for postnatal neural development and is thought to be involved in the process of myelination.

实验方案

Product Specific Protocols
WB protocol for MAG antibody 86097-1-RRDownload protocol
IHC protocol for MAG antibody 86097-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols