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验证数据展示

  • WB analysis using 86064-1-RR (same clone as 86064-1-PBS)

    WB analysis using 86064-1-RR (same clone as 86064-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86064-1-RR (WIBG antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86064-1-PBS in a different storage buffer formulation.

  • WB analysis using 86064-1-RR (same clone as 86064-1-PBS)

    WB analysis using 86064-1-RR (same clone as 86064-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86064-1-RR (WIBG antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86064-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse colon using 86064-1-RR (same clone as 86064-1-PBS)

    IHC staining of mouse colon using 86064-1-RR (same clone as 86064-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 86064-1-RR (WIBG antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86064-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 86064-1-RR (same clone as 86064-1-PBS)

    IF Staining of MCF-7 using 86064-1-RR (same clone as 86064-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using WIBG antibody (86064-1-RR, Clone: 242800G10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86064-1-PBS in a different storage buffer formulation.

  • Cytometric bead array sample test of MP02733-1

    Cytometric bead array sample test of MP02733-1

    The mean WIBG concentration was determined to be 112.9 ng/mL in U2OS cell extract based on a 1.2 mg/mL extract load, 201.7 ng/mL in A549 cell extract based on a 1.2 mg/mL extract load.

  • Cytometric bead array standard curve of MP02733-1

    Cytometric bead array standard curve of MP02733-1

    Cytometric bead array standard curve of MP02733-1, WIBG Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 86064-1-PBS. Detection antibody: 86064-2-PBS. Standard: Ag6276. Range: 1.562-200 ng/mL.

WIBG Recombinant monoclonal antibody, PBS Only (Capture)

WIBG Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 86064-1-PBS

产品说明书

CloneNo. 242800G10

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

别名

Partner of Y14 and mago, Protein wibg homolog, PYM, PYM homolog 1 exon junction complex-associated factor, PYM1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

86064-1-PBS targets WIBG as part of a matched antibody pair:

MP02733-1: 86064-1-PBS capture and 86064-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag6276

Product name: Recombinant human WIBG protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-204 aa of BC006135

Sequence: MEAAGSPAATETGKYIASTQRPDGTWRKQRRVKEGYVPQEEVPVYENKYVKFFKSKPELPPGLSPEATAPVTPSRPEGGEPGLSKTAKRNLKRKEKRRQQQEKGEAEALSRTLDKVSLEETAQLPSAPQGSRAAPTAASDQPDSAATTEKAKKIKNLKKKLRQVEELQQRIQAGEVSQPSKEQLEKLARRRALEEELEDLELGL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 within bgcn homolog (Drosophila)
别名 Partner of Y14 and mago, Protein wibg homolog, PYM, PYM homolog 1 exon junction complex-associated factor, PYM1
计算分子量 23 kDa
观测分子量23-27 kDa
GenBank蛋白编号BC006135
基因名称 WIBG
Gene ID (NCBI) 84305
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9BRP8
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Protein WIBG homolog, also known as PYM1 (Partner of Y14 and mago), is a cytoplasmic RNA-binding protein, that can interact with the ribosome and EJC, may act as an EJC destabilizing factor, preventing EJC accumulation at positions away from exon-exon junctions (non-canonical position).

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