验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, MCF-7 cells, SH-SY5Y cells, HepG2 cells, U-251 cells, HL-60 cells, mouse thymus tissue, mouse spleen tissue |
| Positive IHC detected in | mouse colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
86064-1-RR targets WIBG in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 |
CatNo: Ag6276 Product name: Recombinant human WIBG protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-204 aa of BC006135 Sequence: MEAAGSPAATETGKYIASTQRPDGTWRKQRRVKEGYVPQEEVPVYENKYVKFFKSKPELPPGLSPEATAPVTPSRPEGGEPGLSKTAKRNLKRKEKRRQQQEKGEAEALSRTLDKVSLEETAQLPSAPQGSRAAPTAASDQPDSAATTEKAKKIKNLKKKLRQVEELQQRIQAGEVSQPSKEQLEKLARRRALEEELEDLELGL 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | within bgcn homolog (Drosophila) |
| 别名 | Partner of Y14 and mago, Protein wibg homolog, PYM, PYM homolog 1 exon junction complex-associated factor, PYM1 |
| 计算分子量 | 23 kDa |
| 观测分子量 | 23-27 kDa |
| GenBank蛋白编号 | BC006135 |
| 基因名称 | WIBG |
| Gene ID (NCBI) | 84305 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q9BRP8 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Protein WIBG homolog, also known as PYM1 (Partner of Y14 and mago), is a cytoplasmic RNA-binding protein, that can interact with the ribosome and EJC, may act as an EJC destabilizing factor, preventing EJC accumulation at positions away from exon-exon junctions (non-canonical position).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for WIBG antibody 86064-1-RR | Download protocol |
| IHC protocol for WIBG antibody 86064-1-RR | Download protocol |
| WB protocol for WIBG antibody 86064-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
