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验证数据展示

  • WB analysis using 86064-1-RR

    WB analysis using 86064-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86064-1-RR (WIBG antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis using 86064-1-RR

    WB analysis using 86064-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86064-1-RR (WIBG antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse colon using 86064-1-RR

    IHC staining of mouse colon using 86064-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 86064-1-RR (WIBG antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of MCF-7 using 86064-1-RR

    IF Staining of MCF-7 using 86064-1-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using WIBG antibody (86064-1-RR, Clone: 242800G10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

WIBG Recombinant antibody

WIBG Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 86064-1-RR

产品说明书

CloneNo. 242800G10

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

Partner of Y14 and mago, Protein wibg homolog, PYM, PYM homolog 1 exon junction complex-associated factor, PYM1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA549 cells, MCF-7 cells, SH-SY5Y cells, HepG2 cells, U-251 cells, HL-60 cells, mouse thymus tissue, mouse spleen tissue
Positive IHC detected inmouse colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

86064-1-RR targets WIBG in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 WIBG fusion protein Ag6276 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 within bgcn homolog (Drosophila)
别名 Partner of Y14 and mago, Protein wibg homolog, PYM, PYM homolog 1 exon junction complex-associated factor, PYM1
计算分子量 23 kDa
观测分子量23-27 kDa
GenBank蛋白编号BC006135
基因名称 WIBG
Gene ID (NCBI) 84305
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9BRP8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Protein WIBG homolog, also known as PYM1 (Partner of Y14 and mago), is a cytoplasmic RNA-binding protein, that can interact with the ribosome and EJC, may act as an EJC destabilizing factor, preventing EJC accumulation at positions away from exon-exon junctions (non-canonical position).

实验方案

Product Specific Protocols
WB protocol for WIBG antibody 86064-1-RRDownload protocol
IHC protocol for WIBG antibody 86064-1-RRDownload protocol
IF protocol for WIBG antibody 86064-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols