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验证数据展示

  • WB analysis of human placenta using 86010-2-RR

    WB analysis of human placenta using 86010-2-RR

    human placenta tissue were subjected to SDS PAGE followed by western blot with 86010-2-RR (TEK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of HUVEC using 86010-2-RR

    WB analysis of HUVEC using 86010-2-RR

    HUVEC cells were subjected to SDS PAGE followed by western blot with 86010-2-RR (TEK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Tie-2/CD202b Recombinant antibody

Tie-2/CD202b Recombinant Antibody for WB, ELISA
Cat No. 86010-2-RR

产品说明书

CloneNo. 250234F8

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

TEK, Angiopoietin 1 receptor, Angiopoietin-1 receptor, CD202B, EC:2.7.10.1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inhuman placenta tissue, HUVEC cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

86010-2-RR targets Tie-2/CD202b in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 Tie-2/CD202b fusion protein Eg3367 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 TEK tyrosine kinase, endothelial
别名 TEK, Angiopoietin 1 receptor, Angiopoietin-1 receptor, CD202B, EC:2.7.10.1
计算分子量 1124 aa, 126 kDa
观测分子量120 kDa, 160 kDa
GenBank蛋白编号BC035514
基因名称 Tie2
Gene ID (NCBI) 7010
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ02763
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Tie2 (also known as TEK) is a tyrosine-protein kinase expressed almost exclusively on endothelial cells. It contains two immunoglobulin-like domains, three epidermal growth factor (EGF)--like domains and three fibronectin type III repeats. Tie2 acts as a cell-surface receptor for ANGPT1, ANGPT2, and ANGPT4 and regulates angiogenesis, endothelial cell survival, proliferation, migration, adhesion and cell spreading, reorganization of the actin cytoskeleton, but also maintenance of vascular quiescence. Mutations in the gene Tie2 are associated with inherited venous malformations of the skin and mucous membranes. Human Tie2 has a calculated molecular weight of 126 kDa. As a result of glycosylation, the apparent molecular mass of Tie2 is approximately 140-160 kDa.

实验方案

Product Specific Protocols
WB protocol for Tie-2/CD202b antibody 86010-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols