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验证数据展示

  • WB analysis using 85980-2-RR

    WB analysis using 85980-2-RR

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 85980-2-RR (ALDH1L1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of Neuro-2a using 85980-2-RR

    IF Staining of Neuro-2a using 85980-2-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed Neuro-2a cells using ALDH1L1 antibody (85980-2-RR, Clone: 250565B12 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 85980-2-RR

    Affinity and Kinetic Characterization of 85980-2-RR

    Biolayer interferometry (BLl) kinetic assays of 85980-2-RR against Human ALDH1L1 were performed. The affinity constant is 2.04 nM.

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ALDH1L1 Recombinant monoclonal antibody

ALDH1L1 Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 85980-2-RR

产品说明书

CloneNo. 250565B12

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

10 FTHFDH, 10-FTHFDH, ALDH1L 1, Cytosolic 10-formyltetrahydrofolate dehydrogenase, EC:1.5.1.6

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse liver tissue, rat liver tissue
Positive IF/ICC detected inNeuro-2a cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85980-2-RR targets ALDH1L1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag11285

Product name: Recombinant human ALDH1L1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 157-505 aa of BC027241

Sequence: DDTVSTLYNRFLFPEGIKGMVQAVRLIAEGKAPRLPQPEEGATYEGIQKKETAKINWDQPAEAIHNWIRGNDKVPGAWTEACEQKLTFFNSTLNTSGLVPEGDALPIPGAHRPGVVTKAGLILFGNDDKMLLVKNIQLEDGKMILASNFFKGAASSVLELTEAELVTAEAVRSVWQRILPKVLEVEDSTDFFKSGAASVDVVRLVEEVKELCDGLELENEDVYMASTFGDFIQLLVRKLRGDDEEGECSIDYVEMAVNKRTVRMPHQLFIGGEFVDAEGAKTSETINPTDGSVICQVSLAQVTDVDKAVAAAKDAFENGRWGKISARDRGRLMYRAPPSPSTRPDPTAT

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 aldehyde dehydrogenase 1 family, member L1
别名 10 FTHFDH, 10-FTHFDH, ALDH1L 1, Cytosolic 10-formyltetrahydrofolate dehydrogenase, EC:1.5.1.6
计算分子量 99 kDa
观测分子量100 kDa
GenBank蛋白编号BC027241
基因名称 ALDH1L1
Gene ID (NCBI) 10840
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO75891
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Aldehyde dehydrogenase 1 family member L1 (ALDH1L1), belongs to the aldehyde dehydrogenase family of proteins and is responsible for formate oxidation in vivo. Loss of ALDH1L1 is associated with decreased apoptosis, increased cell motility, and cancer progression. ALDH1L1 catalyzes the conversion of 10-formyltetrahydrofolate, nicotinamide adenine dinucleotide phosphate (NADP+), and water to tetrahydrofolate, NADPH, and carbon dioxide. ALDH1L1 has some isoforms with MW of 99, 88 and 55 kDa.

实验方案

Product Specific Protocols
WB protocol for ALDH1L1 antibody 85980-2-RRDownload protocol
IF protocol for ALDH1L1 antibody 85980-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols