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验证数据展示

  • WB analysis using 85861-1-RR (same clone as 85861-1-PBS)

    WB analysis using 85861-1-RR (same clone as 85861-1-PBS)

    Mouse CCL19 Recombinant protein were subjected to SDS PAGE followed by western blot with 85861-1-RR (Ccl19 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85861-1-PBS in a different storage buffer formulation.

  • WB analysis using 85861-1-RR (same clone as 85861-1-PBS)

    WB analysis using 85861-1-RR (same clone as 85861-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85861-1-RR (Ccl19 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85861-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse spleen using 85861-1-RR (same clone as 85861-1-PBS)

    IHC staining of mouse spleen using 85861-1-RR (same clone as 85861-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 85861-1-RR (CCL19/MIP-3 beta antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85861-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse spleen using 85861-1-RR (same clone as 85861-1-PBS)

    IHC staining of mouse spleen using 85861-1-RR (same clone as 85861-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 85861-1-RR (CCL19/MIP-3 beta antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85861-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA sample test of MP02165-1

    Sandwich ELISA sample test of MP02165-1

    Mouse spleen tissue was removed, rinsed in 1x PBS, and kept on ice. The tissue was then homogenized using a tissue homogenizer and seeded into media containing RPMI 1640 supplemented with 10% fetal bovine serum, 2 mM L-glutamine, 100 U/mL penicillin, and 100 mg/mL streptomycin sulfate. Cells were cultured for 3 days. Aliquots of the cell culture supernatants were removed and assayed for levels of mouse CCL19/MIP-3 beta, and measured 25.7 pg/mL.

  • Sandwich ELISA sample test of MP02165-1

    Sandwich ELISA sample test of MP02165-1

    Serum of four mice was measured. The CCL19/MIP-3 beta concentration of detected samples was determined to be 424.5 pg/mL with a range of 338.8-499.6 pg/mL.

  • Sandwich ELISA standard curve of MP02165-1

    Sandwich ELISA standard curve of MP02165-1

    Sandwich ELISA standard curve of MP02165-1, Mouse CCL19/MIP-3 beta Recombinant Matched Antibody Pair - PBS only. 85861-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg3124. 85861-1-PBS was HRP conjugated as the detection antibody. Range: 12.5-800 pg/mL.

  • Cytometric bead array standard curve of MP02165-1

    Cytometric bead array standard curve of MP02165-1

    Cytometric bead array standard curve of MP02165-1, MOUSE CCL19/MIP-3 beta Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85861-3-PBS. Detection antibody: 85861-1-PBS. Standard: Eg3124. Range: 0.781-100 ng/mL.

Mouse CCL19/MIP-3 beta Recombinant monoclonal antibody, PBS Only (Detector)

CCL19/MIP-3 beta Uni-rAb® Recombinant Antibody for WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA
Cat No. 85861-1-PBS

产品说明书

CloneNo. 242867C1

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA

别名

C-C motif chemokine 19, Ccl19, EBI1 ligand chemokine, ELC, Epstein-Barr virus-induced molecule 1 ligand chemokine

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

85861-1-PBS targets CCL19/MIP-3 beta as part of a matched antibody pair:

MP02165-1: 85861-3-PBS capture and 85861-1-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA Application Description
经测试反应性 mouse
免疫原

CatNo: Eg3124

Product name: Recombinant Mouse CCL19/MIP-3 beta protein (rFc Tag)

Source: mammalian cells-derived, V37

Tag: C-rFc

Domain: 26-108 aa of NM_011888.2

Sequence: GANDAEDCCLSVTQRPIPGNIVKAFRYLLNEDGCRVPAVVFTTLRGYQLCAPPDQPWVDRIIRRLKKSSAKNKGNSTRRSPVS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 chemokine (C-C motif) ligand 19
别名 C-C motif chemokine 19, Ccl19, EBI1 ligand chemokine, ELC, Epstein-Barr virus-induced molecule 1 ligand chemokine
计算分子量12 kDa
GenBank蛋白编号NM_011888.2
基因名称 Ccl19
Gene ID (NCBI) 24047
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO70460
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CCL19, also known as MIP-3β, is one of several CC cytokine genes clustered on the p-arm of chromosome 9. CCL19 may play a role in normal lymphocyte recirculation and homing. It also plays an important role in trafficking of T cells in thymus, and in T cell and B cell migration to secondary lymphoid organs. It specifically binds to chemokine receptor CCR7.

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