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验证数据展示

  • IF Staining of Vero using 85780-2-RR

    IF Staining of Vero using 85780-2-RR

    Immunofluorescent analysis of (4% PFA) fixed Live vaccines of TGEV and PEDV-infected Vero cells using dsRNA antibody (85780-2-RR, Clone: 250708A2 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Dot Blot experiment using 85780-2-RR

    Dot Blot experiment using 85780-2-RR

    Dot blot analysis was used to confirm the specificity of 85780-2-RR anti-dsRNA antibody. samples were spotted onto NC and probed with antibody at 5 µg/ml.The amount of sample (μg) spotted is indicated next to each row.

  • IF Staining of Transfected Vero using 85780-2-RR

    IF Staining of Transfected Vero using 85780-2-RR

    Immunofluorescent analysis of (4% PFA) fixed Transfected Vero using dsRNA antibody (85780-2-RR, Clone: 250708A2 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • FC experiment of Transfected Vero using 85780-2-RR

    FC experiment of Transfected Vero using 85780-2-RR

    1x10^6 Transfected Vero were intracellularly stained with 0.25 ug dsRNA Recombinant antibody (85780-2-RR, Clone:250708A2) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), and 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Dot Blot experiment of Poly(I:C) using 85780-2-RR

    Dot Blot experiment of Poly(I:C) using 85780-2-RR

    Dot blot analysis was used to confirm the specificity of 85780-2-RR dsRNA antibody. samples were spotted onto NC and probed with antibody at 1 µg/ml.The amount of sample (μg/mL) spotted is indicated next to each row.

dsRNA Recombinant antibody

dsRNA Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), Dot Blot, ELISA
Cat No. 85780-2-RR

产品说明书

CloneNo. 250708A2

宿主/亚型

Rabbit / IgG

种属反应性

n/a

应用

IF/ICC, FC (Intra), Dot Blot, ELISA

别名

250708A2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite®488
规格价格库存

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经过测试的应用

Positive IF/ICC detected inLive vaccines of TGEV and PEDV-infected Vero cells, Transfected Vero
Positive FC (Intra) detected inTransfected Vero
Positive Dot Blot detected inN/A, Poly(I:C)

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
DOT BLOTDOT BLOT : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85780-2-RR targets dsRNA in IF/ICC, FC (Intra), Dot Blot, ELISA applications and shows reactivity with n/a samples.

经测试应用 IF/ICC, FC (Intra), Dot Blot, ELISA Application Description
经测试反应性 n/a
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 dsRNA
别名 250708A2
基因名称
Gene ID (NCBI)
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

dsRNA is formed by the base pairing of two RNA strands, creating a double helix structure similar to DNA, but with some differences. dsRNA can be generated through various mechanisms. In the context of viral infections, it can be derived from the genomes of dsRNA viruses, replication intermediates of single-stranded RNA viruses, or bidirectional transcription of certain DNA viruses. Additionally, aberrant splicing or transcriptional processes may lead to the formation of dsRNA structures. dsRNA is recognized as a pathogen-associated molecular pattern (PAMP) by the immune system. It can trigger a series of immune responses in cells, including the activation of antiviral innate immune pathways.

实验方案

Product Specific Protocols
IF protocol for dsRNA antibody 85780-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols