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验证数据展示

  • WB analysis using 85763-4-RR (same clone as 85763-4-PBS)

    WB analysis using 85763-4-RR (same clone as 85763-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85763-4-RR (DDX23 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85763-4-PBS in a different storage buffer formulation.

  • Sandwich ELISA sample test of MP02081-3

    Sandwich ELISA sample test of MP02081-3

    The mean DDX23 concentration was determined to be 263.11 ng/mL in K-562 cell extract based on a 1.20 mg/mL extract load.

  • Sandwich ELISA standard curve of MP02081-3

    Sandwich ELISA standard curve of MP02081-3

    Sandwich ELISA standard curve of MP02081-3, Human DDX23 Recombinant Matched Antibody Pair - PBS only. 85763-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag0258. 85763-2-PBS was HRP conjugated as the detection antibody. Range: 0.781-50 ng/mL.

  • IHC staining of human ovary cancer using 85763-4-RR (same clone as 85763-4-PBS)

    IHC staining of human ovary cancer using 85763-4-RR (same clone as 85763-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 85763-4-RR (DDX23 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85763-4-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with DDX23 antibody included
  • IF Staining of HeLa using 85763-4-RR (same clone as 85763-4-PBS)

    IF Staining of HeLa using 85763-4-RR (same clone as 85763-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DDX23 antibody (85763-4-RR, Clone: 243147B4 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85763-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85763-4-RR

    Affinity and Kinetic Characterization of 85763-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85763-4-RR against Human DDX23 were performed. The affinity constant is below 1 pM.

DDX23 Recombinant monoclonal antibody, PBS Only (Capture)

DDX23 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, Sandwich ELISA, Indirect ELISA
Cat No. 85763-4-PBS

产品说明书

CloneNo. 243147B4

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, Sandwich ELISA, Indirect ELISA

别名

100 kDa U5 snRNP-specific protein, DEAD box protein 23, EC:3.6.4.13, Probable ATP-dependent RNA helicase DDX23, prp28

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy DDX23 Ready-To-Use IHC Kit. DDX23 primary antibody included.

产品信息

85763-4-PBS targets DDX23 as part of a matched antibody pair:

MP02081-3: 85763-4-PBS capture and 85763-2-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF/ICC, Sandwich ELISA, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag0258

Product name: Recombinant human DDX23 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 614-820 aa of BC002366

Sequence: ERLARSYLRRPAVVYIGSAGKPHERVEQKVFLMSESEKRKKLLAILEQGFDPPIIIFVNQKKGCDVLAKSLEKMGYNACTLHGGKGQEQREFALSNLKAGAKDILVATDVAGRGIDIQDVSMVVNYDMAKNIEDYIHRIGRTGRAGKSGVAITFLTKEDSAVFYELKQAILESPVSSCPPELANHPDAQHKPGTILTKKRREETIFA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 DEAD (Asp-Glu-Ala-Asp) box polypeptide 23
别名 100 kDa U5 snRNP-specific protein, DEAD box protein 23, EC:3.6.4.13, Probable ATP-dependent RNA helicase DDX23, prp28
计算分子量 96 kDa
观测分子量96-100 kDa
GenBank蛋白编号BC002366
基因名称 DDX23
Gene ID (NCBI) 9416
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9BUQ8
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DEAD (Asp-Glu-Ala-Asp) box polypeptide 23 (DDX23, synonyms: prp28, MGC8416, U5-100K) is a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. DDX23 is a component of the U5 snRNP complex; it may facilitate conformational changes in the spliceosome during nuclear pre-mRNA splicing.

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