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验证数据展示

  • WB analysis using 85763-4-RR

    WB analysis using 85763-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85763-4-RR (DDX23 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 85763-4-RR

    IF Staining of HeLa using 85763-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DDX23 antibody (85763-4-RR, Clone: 243147B4 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 85763-4-RR

    Affinity and Kinetic Characterization of 85763-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85763-4-RR against Human DDX23 were performed. The affinity constant is below 1 pM.

DDX23 Recombinant antibody

DDX23 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 85763-4-RR

产品说明书

CloneNo. 243147B4

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, ELISA

别名

100 kDa U5 snRNP-specific protein, DEAD box protein 23, EC:3.6.4.13, Probable ATP-dependent RNA helicase DDX23, prp28

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inCOLO 320 cells, HeLa cells, HEK-293T cells, K-562 cells, A549 cells, NIH/3T3 cells, fetal human brain tissue
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy DDX23 Ready-To-Use IHC Kit. DDX23 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85763-4-RR targets DDX23 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 DDX23 fusion protein Ag0258 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 DEAD (Asp-Glu-Ala-Asp) box polypeptide 23
别名 100 kDa U5 snRNP-specific protein, DEAD box protein 23, EC:3.6.4.13, Probable ATP-dependent RNA helicase DDX23, prp28
计算分子量 96 kDa
观测分子量96-100 kDa
GenBank蛋白编号BC002366
基因名称 DDX23
Gene ID (NCBI) 9416
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9BUQ8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DEAD (Asp-Glu-Ala-Asp) box polypeptide 23 (DDX23, synonyms: prp28, MGC8416, U5-100K) is a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. DDX23 is a component of the U5 snRNP complex; it may facilitate conformational changes in the spliceosome during nuclear pre-mRNA splicing.

实验方案

Product Specific Protocols
WB protocol for DDX23 antibody 85763-4-RRDownload protocol
IF protocol for DDX23 antibody 85763-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols