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验证数据展示

  • WB analysis of human saliva using 85745-3-RR (same clone as 85745-3-PBS)

    WB analysis of human saliva using 85745-3-RR (same clone as 85745-3-PBS)

    human saliva were subjected to SDS PAGE followed by western blot with 85745-3-RR (SMR3B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85745-3-PBS in a different storage buffer formulation.

  • Sandwich ELISA sample test of MP02114-1

    Sandwich ELISA sample test of MP02114-1

    The mean SMR3B concentration was determined to be 8.50 ng/mL in HeLa cell extract based on a 1.30 mg/mL extract load, 8.00 ng/mL in HepG2 cell extract based on a 0.90 mg/mL extract load, 12.70 ng/mL in A549 cell extract based on a 2.40 mg/mL extract load and 9.20 ng/mL in MCF-7 cell extract based on a 1.20 mg/mL extract load.

  • Sandwich ELISA standard curve of MP02114-1

    Sandwich ELISA standard curve of MP02114-1

    Sandwich ELISA standard curve of MP02114-1, Human SMR3B Recombinant Matched Antibody Pair - PBS only. 85745-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg3081. 85745-2-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL.

  • Cytometric bead array standard curve of MP02114-1

    Cytometric bead array standard curve of MP02114-1

    Cytometric bead array standard curve of MP02114-1, SMR3B Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85745-3-PBS. Detection antibody: 85745-2-PBS. Standard: Eg3081. Range: 0.313-40 ng/mL.

  • Affinity of 85745-3-RR

    Affinity of 85745-3-RR

    Biolayer interferometry (BLl) kinetic assays of 85745-3-RR against Human SMR3B were performed. The affinity constant is 0.785 nM.

SMR3B Recombinant monoclonal antibody, PBS Only (Capture)

SMR3B Uni-rAb® Recombinant Antibody for WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA
Cat No. 85745-3-PBS

产品说明书

CloneNo. 242868D3

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA

别名

P B, PBII, PRL3, PROL3, Proline rich peptide P B

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

85745-3-PBS targets SMR3B as part of a matched antibody pair:

MP02114-1: 85745-3-PBS capture and 85745-2-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Eg3081

Product name: Recombinant Human SMR3B protein (rFc Tag) (HPLC verified)

Source: mammalian cells-derived, V37

Tag: C-rFc

Domain: 23-79 aa of NM_006685.4

Sequence: QRGPRGPYPPGPLAPPQPFGPGFVPPPPPPPYGPGRIPPPPPAPYGPGIFPPPPPQP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 submaxillary gland androgen regulated protein 3B
别名 P B, PBII, PRL3, PROL3, Proline rich peptide P B
计算分子量8 kDa
观测分子量8 kDa
GenBank蛋白编号NM_006685.4
基因名称 SMR3B
Gene ID (NCBI) 10879
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP02814
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SMR3B is an androgen-regulated secreted PRP-family peptide produced primarily in the submandibular and sublingual glands. Released into saliva, it contributes to lubrication, oral defense, and glandular homeostasis. Highly tissue-specific, SMR3B serves as a marker of salivary gland function and is altered in xerostomia, Sjögren's syndrome, and head-and-neck cancers, highlighting its relevance in secretory physiology and disease.

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