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验证数据展示

  • WB analysis using 85732-2-RR (same clone as 85732-2-PBS)

    WB analysis using 85732-2-RR (same clone as 85732-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85732-2-RR (A2BP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85732-2-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 85732-2-RR (same clone as 85732-2-PBS)

    IHC staining of mouse brain using 85732-2-RR (same clone as 85732-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 85732-2-RR (A2BP1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85732-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RBFOX1/A2BP1 antibody included
  • IHC staining of mouse brain using 85732-2-RR (same clone as 85732-2-PBS)

    IHC staining of mouse brain using 85732-2-RR (same clone as 85732-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 85732-2-RR (A2BP1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85732-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RBFOX1/A2BP1 antibody included
  • IF Staining of mouse brain using 85732-2-RR (same clone as 85732-2-PBS)

    IF Staining of mouse brain using 85732-2-RR (same clone as 85732-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using A2BP1 antibody (85732-2-RR, Clone: 243149B4 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85732-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP02075-1

    Cytometric bead array standard curve of MP02075-1

    Cytometric bead array standard curve of MP02075-1, A2BP1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85732-2-PBS. Detection antibody: 85732-4-PBS. Standard: Ag18527. Range: 0.469-60 ng/mL.

  • Affinity and Kinetic Characterization of 85732-2-RR

    Affinity and Kinetic Characterization of 85732-2-RR

    Biolayer interferometry (BLl) kinetic assays of 85732-2-RR against Human A2BP1 were performed. The affinity constant is 2.82 nM.

A2BP1 Recombinant monoclonal antibody, PBS Only (Capture)

A2BP1 Uni-rAb® Recombinant Antibody for WB, IHC, IF-P, Cytometric bead array, Indirect ELISA
Cat No. 85732-2-PBS

产品说明书

CloneNo. 243149B4

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

别名

FOX1, RBFOX1, A2BP, Ataxin-2-binding protein 1, Fox-1 homolog A

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy RBFOX1/A2BP1 Ready-To-Use IHC Kit. RBFOX1/A2BP1 primary antibody included.

产品信息

85732-2-PBS targets A2BP1 as part of a matched antibody pair:

MP02075-1: 85732-2-PBS capture and 85732-4-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF-P, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag18527

Product name: Recombinant human A2BP1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-128 aa of BC113691

Sequence: MLASQGVLLHPYGVPMIVPAAPYLPGLIQGNQEAAAAPDTMAQPYASAQFAPPQNGIPAEYTAPHPHPAPEYTGQTTVPEHTLNLYPPAQTHSEQSPADTSAQTVSGTATQTDDAAPTDGQPQTQPSE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ataxin 2-binding protein 1
别名 FOX1, RBFOX1, A2BP, Ataxin-2-binding protein 1, Fox-1 homolog A
计算分子量 395 aa, 42 kDa
观测分子量 45-60 kDa
GenBank蛋白编号BC113691
基因名称 A2BP1
Gene ID (NCBI) 54715
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9NWB1
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

A2BP1, also named as FOX1 and HRNBP1, contains one RRM (RNA recognition motif) domain. A2BP1 recognizes a (U)GCAUG stretch in regulated exons or in flanking introns. The protein binds to the C-terminus of ataxin-2 and may contribute to the restricted pathology of spinocerebellar ataxia type 2 (SCA2). Ataxin-2 is the product of the SCA2 gene which causes familial neurodegenerative diseases. A2BP1 and ataxin-2 are both localized in the trans-Golgi network.

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