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验证数据展示

  • WB analysis using 85681-4-RR

    WB analysis using 85681-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85681-4-RR (KTN1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 85681-4-RR

    IHC staining of human ovary cancer using 85681-4-RR

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 85681-4-RR (KTN1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KTN1 antibody included
  • IHC staining of human ovary cancer using 85681-4-RR

    IHC staining of human ovary cancer using 85681-4-RR

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 85681-4-RR (KTN1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KTN1 antibody included
  • IF Staining of HepG2 using 85681-4-RR

    IF Staining of HepG2 using 85681-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using KTN1 antibody (85681-4-RR, Clone: 243067A11 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

KTN1 Recombinant antibody

KTN1 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 85681-4-RR

产品说明书

CloneNo. 243067A11

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

CG-1 antigen, KIAA0004, KTN 1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, Jurkat cells, K-562 cells, PC-3 cells, SKOV-3 cells, A549 cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy KTN1 Ready-To-Use IHC Kit. KTN1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85681-4-RR targets KTN1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 KTN1 fusion protein Ag13854 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 kinectin 1 (kinesin receptor)
别名 CG-1 antigen, KIAA0004, KTN 1
计算分子量 1357 aa, 156 kDa
观测分子量 156 kDa
GenBank蛋白编号BC117132
基因名称 KTN1
Gene ID (NCBI) 3895
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ86UP2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

KTN1/Kinectin is an integral endoplasmic reticulum (ER) membrane protein which acts as dynamic anchor to facilitate ER membrane extension along microtubules. It has been found to bind kinesin, Rho GTPase, and translation elongation factor-1delta and gets involved in organelle motility and protein synthesis.

实验方案

Product Specific Protocols
WB protocol for KTN1 antibody 85681-4-RRDownload protocol
IHC protocol for KTN1 antibody 85681-4-RRDownload protocol
IF protocol for KTN1 antibody 85681-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols