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验证数据展示

  • WB analysis using 85634-2-RR (same clone as 85634-2-PBS)

    WB analysis using 85634-2-RR (same clone as 85634-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85634-2-RR (ST3GAL2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85634-2-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 85634-2-RR (same clone as 85634-2-PBS)

    IHC staining of human colon cancer using 85634-2-RR (same clone as 85634-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 85634-2-RR (ST3GAL2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85634-2-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 85634-2-RR (same clone as 85634-2-PBS)

    IHC staining of human colon cancer using 85634-2-RR (same clone as 85634-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 85634-2-RR (ST3GAL2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85634-2-PBS in a different storage buffer formulation.

  • Cytometric bead array sample test of MP03241-1

    Cytometric bead array sample test of MP03241-1

    The mean ST3GAL2 concentration was determined to be 15.8 ng/mL in HT-29 cell extract based on a 1.2 mg/mL extract load, 0.5 ng/mL in A431 cell extract based on a 1.2 mg/mL extract load.

  • Cytometric bead array standard curve of MP03241-1

    Cytometric bead array standard curve of MP03241-1

    Cytometric bead array standard curve of MP03241-1, ST3GAL2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85634-3-PBS. Detection antibody: 85634-2-PBS. Standard: Ag4528. Range: 1.562-200 ng/mL.

ST3GAL2 Recombinant monoclonal antibody, PBS Only (Detector)

ST3GAL2 Uni-rAb® Recombinant Antibody for WB, IHC, Cytometric bead array, Indirect ELISA
Cat No. 85634-2-PBS

产品说明书

CloneNo. 253479G5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, Cytometric bead array, Indirect ELISA

别名

Alpha 2,3-ST 2, Beta-galactoside alpha-2,3-sialyltransferase 2, CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,3-sialyltransferase 2, EC:2.4.3.2, EC:2.4.3.4

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

85634-2-PBS targets ST3GAL2 as part of a matched antibody pair:

MP03241-1: 85634-3-PBS capture and 85634-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag4528

Product name: Recombinant human ST3GAL2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 27-210 aa of BC036777

Sequence: SHHSMATLPYLDSGALDGTHRVKLVPGYAGLQRLSKERLSGKSCACRRCMGDAGASDWFDSHFDGNISPVWTRENMDLPPDVQRWWMMLQPQFKSHNTNEVLEKLFQIVPGENPYRFRDPHQCRRCAVVGNSGNLRGSGYGQDVDGHNFIMRMNQAPTVGFEQDVGSRTTHHFMYPESAKNLPA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ST3 beta-galactoside alpha-2,3-sialyltransferase 2
别名 Alpha 2,3-ST 2, Beta-galactoside alpha-2,3-sialyltransferase 2, CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,3-sialyltransferase 2, EC:2.4.3.2, EC:2.4.3.4
计算分子量 350 aa, 40 kDa
观测分子量40-43 kDa
GenBank蛋白编号BC036777
基因名称 ST3GAL2
Gene ID (NCBI) 6483
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ16842
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Beta-galactoside alpha-2,3-sialyltransferase 2 (ST3GAL2) is a sialyltransferase (ST) that transfers sialic acid from the activated donor CMP-sialic acid to the glycan terminus of a glycolipid or glycoprotein. ST3GAL2 contains two putative N-glycosylation sites (Asn92 and Asn211), which are mainly responsible for GD1a and GT1b ganglioside biosynthesis in the brain. ST3GAL2 belongs to the sialyltransferase family, which mediates the terminal modification of glycoproteins and glycolipids. ST3GAL2 has been found to play a role in obesity, aging, and malignant diseases (PMID: 34319453).

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