• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
Use Able AI chat for product recommendations

验证数据展示

  • WB analysis using 85449-5-RR

    WB analysis using 85449-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85449-5-RR (RIOK3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 85449-5-RR

    IHC staining of human breast cancer using 85449-5-RR

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 85449-5-RR (RIOK3 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 85449-5-RR

    IHC staining of human breast cancer using 85449-5-RR

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 85449-5-RR (RIOK3 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human rectal cancer using 85449-5-RR

    IHC staining of human rectal cancer using 85449-5-RR

    Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 85449-5-RR (RIOK3 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of Jurkat using 85449-5-RR

    IF Staining of Jurkat using 85449-5-RR

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using RIOK3 antibody (85449-5-RR, Clone: 242996A6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • IF Staining of MCF-7 using 85449-5-RR

    IF Staining of MCF-7 using 85449-5-RR

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using RIOK3 antibody (85449-5-RR, Clone: 242996A6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 85449-5-RR

    Affinity and Kinetic Characterization of 85449-5-RR

    Biolayer interferometry (BLl) kinetic assays of 85449-5-RR against Human RIOK3 were performed. The affinity constant is below 1 pM.

RIOK3 Recombinant monoclonal antibody

RIOK3 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 85449-5-RR

产品说明书

CloneNo. 242996A6

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

242996A6, EC:2.7.11.1, RIO kinase 3, RIO kinase 3 (yeast), Serine/threonine-protein kinase RIO3

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inJurkat cells, MCF-7 cells, HeLa cells, HEK-293T cells, HepG2 cells
Positive IHC detected inhuman rectal cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inJurkat cells, A431 cells, MCF-7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85449-5-RR targets RIOK3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag4497

Product name: Recombinant human RIOK3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 215-516 aa of BC039729

Sequence: RRSARLHEKKEHSTAEKAVDPKTRLLMYKMVNSGMLETITGCISTGKESVVFHAYGGSMEDEKEDSKVIPTECAIKVFKTTLNEFKNRDKYIKDDFRFKDRFSKLNPRKIIRMWAEKEMHNLARMQRAGIPCPTVVLLKKHILVMSFIGHDQVPAPKLKEVKLNSEEMKEAYYQTLHLMRQLYHECTLVHADLSEYNMLWHAGKVWLIDVSQSVEPTHPHGLEFLFRDCRNVSQKGGVKEALSERELFNAVSGLNITADNEADFLAEIEALEKMNEDHVQKNGRKAASFLKDDGDPPLLYDE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 RIO kinase 3 (yeast)
别名 242996A6, EC:2.7.11.1, RIO kinase 3, RIO kinase 3 (yeast), Serine/threonine-protein kinase RIO3
计算分子量 516 aa, 60 kDa
观测分子量60-65 kDa
GenBank蛋白编号BC039729
基因名称 RIOK3
Gene ID (NCBI) 8780
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO14730
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for RIOK3 antibody 85449-5-RRDownload protocol
IHC protocol for RIOK3 antibody 85449-5-RRDownload protocol
WB protocol for RIOK3 antibody 85449-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
||
New chat Able
正在加载，请稍候...