• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
Use Able AI chat for product recommendations

验证数据展示

  • WB analysis using 85438-4-RR

    WB analysis using 85438-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85438-4-RR (CHGB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 85438-4-RR

    WB analysis using 85438-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85438-4-RR (CHGB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas using 85438-4-RR

    IHC staining of human pancreas using 85438-4-RR

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 85438-4-RR (CHGB antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CHGB antibody included
  • IHC staining of human pancreas using 85438-4-RR

    IHC staining of human pancreas using 85438-4-RR

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 85438-4-RR (CHGB antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CHGB antibody included
  • Affinity and Kinetic Characterization of 85438-4-RR

    Affinity and Kinetic Characterization of 85438-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85438-4-RR against Human CHGB were performed. The affinity constant is 1.95 nM.

CHGB Recombinant monoclonal antibody

CHGB Uni-rAb® Recombinant Antibody for WB, IHC, ELISA
Cat No. 85438-4-RR

产品说明书

CloneNo. 242952C9

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

CgB, Chromogranin B, SCG1, 242952C9, Chromogranin-B

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inHeLa cells, BxPC-3 cells, SH-SY5Y cells, RAW 264.7 cells, PC-12 cells, HEK-293T cells
Positive IHC detected inhuman pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy CHGB Ready-To-Use IHC Kit. CHGB primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85438-4-RR targets CHGB in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag6833

Product name: Recombinant human CHGB protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 328-677 aa of BC000375

Sequence: HSTHYRASEEEPEYGEEIKGYPGVQAPEDLEWERYRGRGSEEYRAPRPQSEESWDEEDKRNYPSLELDKMAHGYGEESEEERGLEPGKGRHHRGRGGEPRAYFMSDTREEKRFLGEGHHRVQENQMDKARRHPQGAWKELDRNYLNYGEEGAPGKWQQQGDLQDTKENREEARFQDKQYSSHHTAEKRKRLGELFNPYYDPLQWKSSHFERRDNMNDNFLEGEEENELTLNEKNFFPEYNYDWWEKKPFSEDVNWGYEKRNLARVPKLDLKRQYDRVAQLDQLLHYRKKSAEFPDFYDSEEPVSTHQEAENEKDRADQTVLTEDEKKELENLAAMDLELQKIAEKFSQRG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 chromogranin B (secretogranin 1)
别名 CgB, Chromogranin B, SCG1, 242952C9, Chromogranin-B
计算分子量 78 kDa
观测分子量 70-100 kDa
GenBank蛋白编号BC000375
基因名称 Chromogranin B
Gene ID (NCBI) 1114
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP05060
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Chromogranin B is also named as Secretogranin-1, SgI, CgB, CHGB and SCG1. CHGB, a secretory granule protein, inserts itself into membrane and forms a chloride-conducting channel (PMID: 30456382). Native CHGB distributes nearly equally in soluble and membrane-bound forms, and both reconstitute highly selective anion channels in membrane (PMID: 37435575). The calculated molecular weight of Chromogranin B is at 78kDa.

实验方案

Product Specific Protocols
IHC protocol for CHGB antibody 85438-4-RRDownload protocol
WB protocol for CHGB antibody 85438-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
||
New chat Able
正在加载，请稍候...