验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, BxPC-3 cells, SH-SY5Y cells, RAW 264.7 cells, PC-12 cells, HEK-293T cells |
Planning an IHC experiment? We recommend our IHCeasy CHGB Ready-To-Use IHC Kit. CHGB primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
85438-4-RR targets CHGB in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | CHGB fusion protein Ag6833 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | chromogranin B (secretogranin 1) |
| 别名 | CgB, Chromogranin B, SCG1, 242952C9, Chromogranin-B |
| 计算分子量 | 78 kDa |
| 观测分子量 | 70-100 kDa |
| GenBank蛋白编号 | BC000375 |
| 基因名称 | Chromogranin B |
| Gene ID (NCBI) | 1114 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P05060 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Chromogranin B is also named as Secretogranin-1, SgI, CgB, CHGB and SCG1. CHGB, a secretory granule protein, inserts itself into membrane and forms a chloride-conducting channel (PMID: 30456382). Native CHGB distributes nearly equally in soluble and membrane-bound forms, and both reconstitute highly selective anion channels in membrane (PMID: 37435575). The calculated molecular weight of Chromogranin B is at 78kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CHGB antibody 85438-4-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |