• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis of human placenta using 85182-1-RR

    WB analysis of human placenta using 85182-1-RR

    Human placenta tissue was subjected to SDS PAGE, followed by western blot with 85182-1-RR (GYPA antibody) at a dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 85182-1-RR

    IHC staining of human placenta using 85182-1-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 85182-1-RR (Glycophorin A/CD235a antibody) at dilution of 1:20000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Glycophorin A antibody included
  • IHC staining of human placenta using 85182-1-RR

    IHC staining of human placenta using 85182-1-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 85182-1-RR (Glycophorin A/CD235a antibody) at dilution of 1:20000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Glycophorin A antibody included

Glycophorin A/CD235a Recombinant monoclonal antibody

Glycophorin A/CD235a Uni-rAb® Recombinant Antibody for WB, IHC, ELISA
Cat No. 85182-1-RR

产品说明书

CloneNo. 242822C9

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

Glycophorin A, 242822C9, CD235a, Glycophorin-A, GPA

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inhuman placenta tissue
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy Glycophorin A Ready-To-Use IHC Kit. Glycophorin A primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:10000-1:40000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85182-1-RR targets Glycophorin A/CD235a in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Eg2336

Product name: Recombinant Human Glycophorin A protein (rFc Tag)(HPLC verified)

Source: mammalian cells-derived, pHZ-KIsec-C-rFc

Tag: C-rFc

Domain: 20-91 aa of BC005319

Sequence: SSTTGVAMHTSTSSSVTKSYISSQTNDTHKRDTYAATPRAHEVSEISVRTVYPPEEETGERVQLAHHFSEPE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 glycophorin A (MNS blood group)
别名 Glycophorin A, 242822C9, CD235a, Glycophorin-A, GPA
计算分子量 150 aa, 16 kDa
观测分子量35-38 kDa
GenBank蛋白编号BC005319
基因名称 Glycophorin A
Gene ID (NCBI) 2993
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP02724
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Glycophorin A (GYPA), also known as CD235a, is the major transmembrane sialoglycoprotein in erythrocytes. It is a dimeric type I transmembrane protein carrying 15 closely clustered O-linked tetrasaccharides capped with sialic acid/N-acetylneuraminic acid (Neu5Ac). Glycophorin A represents the major sialoglycoprotein of the red blood cell membrane displaying about one million copies per cell. (PMID: 9490702)

实验方案

Product Specific Protocols
WB protocol for Glycophorin A/CD235a antibody 85182-1-RRDownload protocol
IHC protocol for Glycophorin A/CD235a antibody 85182-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols