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验证数据展示

  • ChIP experiment of K-562 using 85113-4-RR

    ChIP experiment of K-562 using 85113-4-RR

    Chromatin was prepared from K-562 cells. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 15 µg of cross-linked chromatin, 5 µg of ETS2 (85113-4-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR.

  • IF Staining of HeLa using 85113-4-RR

    IF Staining of HeLa using 85113-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ETS2 antibody (85113-4-RR, Clone: 242817H8 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • IF Staining of MCF-7 using 85113-4-RR

    IF Staining of MCF-7 using 85113-4-RR

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using ETS2 antibody (85113-4-RR, Clone: 242817H8 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • FC experiment of MCF-7 using 85113-4-RR

    FC experiment of MCF-7 using 85113-4-RR

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug ETS2 Recombinant antibody (85113-4-RR, Clone:242817H8) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 85113-4-RR

    Affinity and Kinetic Characterization of 85113-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85113-4-RR against Human ETS2 were performed. The affinity constant is below 1 pM.

ETS2 Recombinant monoclonal antibody

ETS2 Uni-rAb® Recombinant Antibody for IF/ICC, FC (Intra), ELISA, ChIP-qPCR
Cat No. 85113-4-RR

产品说明书

CloneNo. 242817H8

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra), ELISA, ChIP-qPCR

别名

242817H8, ETS-2, ETS2IT1, Protein C ets 2, Protein C-ets-2

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IF/ICC detected inHeLa cells, MCF-7 cells
Positive FC (Intra) detected inMCF-7 cells
Positive ChIP-qPCR detected inK-562 cells
Planning an IHC experiment? We recommend our IHCeasy ETS2 Ready-To-Use IHC Kit. ETS2 primary antibody included.

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
CHIP-QPCRCHIP-QPCR : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85113-4-RR targets ETS2 in IF/ICC, FC (Intra), ELISA, ChIP-qPCR applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra), ELISA, ChIP-qPCR Application Description
经测试反应性 human
免疫原

CatNo: Ag2929

Product name: Recombinant human ETS2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 84-444 aa of BC017040

Sequence: KATFSGFKKEQRRLGIPKNPWLWSEQQVCQWLLWATNEFSLVNVNLQRFGMNGQMLCNLGKERFLELAPDFVGDILWEHLEQMIKENQEKTEDQYEENSHLTSVPHWINSNTLGFGTEQAPYGMQTQNYPKGGLLDSMCPASTPSVLSSEQEFQMFPKSRLSSVSVTYCSVSQDFPGSNLNLLTNNSGTPKDHDSPENGADSFESSDSLLQSWNSQSSLLDVQRVPSFESFEDDCSQSLCLNKPTMSFKDYIQERSDPVEQGKPVIPAAVLAGFTGSGPIQLWQFLLELLSDKSCQSFISWTGDGWEFKLADPDEVARRWGKRKNKPKMNYEKLSRGLRYYYDKNIIHKTSGKRYVYRFVC

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 v-ets erythroblastosis virus E26 oncogene homolog 2 (avian)
别名 242817H8, ETS-2, ETS2IT1, Protein C ets 2, Protein C-ets-2
计算分子量 469 aa, 53 kDa
GenBank蛋白编号BC017040
基因名称 ETS2
Gene ID (NCBI) 2114
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP15036
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
FC protocol for ETS2 antibody 85113-4-RRDownload protocol
IF protocol for ETS2 antibody 85113-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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