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验证数据展示

  • WB analysis using 85067-5-RR

    WB analysis using 85067-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85067-5-RR (ARR3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse eye using 85067-5-RR

    IF Staining of mouse eye using 85067-5-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using Arrestin C antibody (85067-5-RR, Clone: 242751B12 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse eye using 85067-5-RR

    IF Staining of mouse eye using 85067-5-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using Arrestin C antibody (85067-5-RR, Clone: 242751B12 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 85067-5-RR

    Affinity and Kinetic Characterization of 85067-5-RR

    Biolayer interferometry (BLl) kinetic assays of 85067-5-RR against Human Arrestin C were performed. The affinity constant is below 1 pM.

Arrestin C Recombinant antibody

Arrestin C Uni-rAbTM Recombinant Antibody for WB, IF-P, ELISA
Cat No. 85067-5-RR

产品说明书

CloneNo. 242751B12

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF-P, ELISA

别名

Arrestin-C, ARR3, C-arrestin, CAR, ARRX

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse eye tissue, rat eye tissues
Positive IF-P detected inmouse eye tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)-PIF-P : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85067-5-RR targets Arrestin C in WB, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF-P, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Arrestin C fusion protein Ag1580 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 arrestin 3, retinal (X-arrestin)
别名 Arrestin-C, ARR3, C-arrestin, CAR, ARRX
计算分子量 43 kDa
观测分子量47-50 kDa
GenBank蛋白编号BC012096
基因名称 Arrestin C
Gene ID (NCBI) 407
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP36575
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Arrestin C, also known as Arrestin 3 or Retinal Cone Arrestin, is a protein encoded by the ARR3 gene. It belongs to the arrestin family, which plays a crucial role in regulating G-protein-coupled receptor (GPCR) signaling and trafficking. Arrestin C is composed of two major domains: the N-domain and the C-domain, connected by a hinge region. These domains form a structure resembling two clamshells placed end-to-end. The C-terminal tail (C-tail) of Arrestin C interacts extensively with the N-domain, stabilizing its basal conformation. Arrestin C is predominantly expressed in cone photoreceptors and pinealocytes in the retina. It is involved in the shut-off mechanisms associated with high-acuity color vision by binding to phosphorylated and activated opsins, thereby inhibiting their ability to interact with transducin.

实验方案

Product Specific Protocols
WB protocol for Arrestin C antibody 85067-5-RRDownload protocol
IF protocol for Arrestin C antibody 85067-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols