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验证数据展示

  • WB analysis using 85063-4-RR

    WB analysis using 85063-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85063-4-RR (BAG2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 85063-4-RR

    WB analysis using 85063-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85063-4-RR (BAG2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 85063-4-RR

    Affinity and Kinetic Characterization of 85063-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85063-4-RR against Human BAG2 were performed. The affinity constant is 0.644 nM.

BAG2 Recombinant antibody

BAG2 Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 85063-4-RR

产品说明书

CloneNo. 242717G4

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, ELISA

别名

242717G4, BAG-2, KIAA0576

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, U-87 MG cells, HeLa cells, HepG2 cells, U-251 cells, mouse testis tissue, rat testis tissue
Planning an IHC experiment? We recommend our IHCeasy BAG2 Ready-To-Use IHC Kit. BAG2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85063-4-RR targets BAG2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 BAG2 fusion protein Ag30873 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 BCL2-associated athanogene 2
别名 242717G4, BAG-2, KIAA0576
计算分子量 24 kDa
观测分子量25 kDa
GenBank蛋白编号NM_004282
基因名称 BAG2
Gene ID (NCBI) 9532
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO95816
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BAG2 (BAG family molecular chaperone regulator 2) is one of six proteins in mammals that contain the BAG domain, which belongs to the BAG (Bcl-2-associated athanogene) family. BAG2 has been described as a negative regulator of the chaperone-associated ubiquitin ligase C terminus of Hsc70-interacting protein (CHIP) that participates in the ubiquitin-mediated proteasomal degradation of misfolded substrate proteins (PMID: 16207813). BAG2 is widely expressed in human tissues, including brown adipose, heart and lung tissue, as well as in various types of tumor cells, including renal cell carcinoma, glioblastoma and thyroid carcinoma cells (PMID: 28536620). BAG2 overexpression is associated with poor prognosis in patients and mutp53 accumulation in tumors (PMID: 26271008).

实验方案

Product Specific Protocols
WB protocol for BAG2 antibody 85063-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols