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验证数据展示

  • Sandwich ELISA standard curve of MP01737-1

    Sandwich ELISA standard curve of MP01737-1

    Sandwich ELISA standard curve of MP01737-1, Human eNOS Recombinant Matched Antibody Pair - PBS only. 85020-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag25712. 85020-2-PBS was HRP conjugated as the detection antibody. Range: 97.7-6250 pg/mL.

  • Sample test of MP01737-1

    Sample test of MP01737-1

    The mean eNOS concentration was determined to be 2.3 ng/mL in bEnd.3 cell extract based on a 1.3 mg/mL extract load and 6.1 ng/mL in THP-1 cell extract based on a 1.5 mg/mL extract load.

  • Cytometric bead array standard curve of MP01737-1

    Cytometric bead array standard curve of MP01737-1

    Cytometric bead array standard curve of MP01737-1, NOS3 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85020-1-PBS. Detection antibody: 85020-2-PBS. Standard: Ag25712. Range: 0.313-40 ng/mL.

  • Affinity and Kinetic Characterization of 85020-2-PBS

    Affinity and Kinetic Characterization of 85020-2-PBS

    Biolayer interferometry (BLl) kinetic assay of 85020-2-PBS against Human NOS3 was performed. The affinity constant is 1.93 nM.

NOS3 Recombinant monoclonal antibody, PBS Only (Detector)

NOS3 Uni-rAb® Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 85020-2-PBS

产品说明书

CloneNo. 242543B3

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

别名

eNOS, cNOS, EC:1.14.13.39, ECNOS, EC-NOS

缓冲液配方:  PBS Only
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

85020-2-PBS targets NOS3 as part of a matched antibody pair:

MP01737-1: 85020-1-PBS capture and 85020-2-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 human
免疫原

CatNo: Ag25712

Product name: Recombinant human NOS3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-120 aa of

Sequence: MGNLKSVAQEPGPPCGLGLGLGLGLCGKQGPATPAPEPSRAPASLLPPAPEHSPPSSPLTQPPEGPKFPRVKNWEVGSITYDTLSAQAQQDGPCTPRRCLGSLVFPRKLQGRPSPGPPAP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 nitric oxide synthase 3 (endothelial cell)
别名 eNOS, cNOS, EC:1.14.13.39, ECNOS, EC-NOS
基因名称 eNOS
Gene ID (NCBI) 4846
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP29474
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C
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