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验证数据展示

  • WB analysis using 84990-4-RR

    WB analysis using 84990-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84990-4-RR (TBC1D5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 84990-4-RR

    IF Staining of A431 using 84990-4-RR

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using TBC1D5 antibody (84990-4-RR, Clone: 242534B8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 84990-4-RR

    Affinity and Kinetic Characterization of 84990-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84990-4-RR against Human TBC1D5 were performed. The affinity constant is 3.28 nM.

TBC1D5 Recombinant antibody

TBC1D5 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 84990-4-RR

产品说明书

CloneNo. 242534B8

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

242534B8, KIAA0210, TBC1 domain family member 5, TBC1 domain family, member 5

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells, PC-3 cells, A431 cells
Positive IF/ICC detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy TBC1D5 Ready-To-Use IHC Kit. TBC1D5 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84990-4-RR targets TBC1D5 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 TBC1D5 fusion protein Ag10991 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 TBC1 domain family, member 5
别名 242534B8, KIAA0210, TBC1 domain family member 5, TBC1 domain family, member 5
计算分子量 795 aa, 89 kDa
观测分子量 89 kDa
GenBank蛋白编号BC013145
基因名称 TBC1D5
Gene ID (NCBI) 9779
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ92609
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TBC1D5, a member of TBC (Tre2/Bub2/Cdc16)1 domain family, is a novel retromer-interacting protein. TBC1D5 is a Rab GTPase-activating proteins (GAPs) proteins that negatively regulates VPS35/29/26 recruitment and causes Rab7 to dissociate from the membrane. TBC1D5 could bridge the endosome and autophagosome via its C-terminal LIR motif, and Rab GAPs are implicated in the reprogramming of the endocytic trafficking events under starvation-induced autophagy. The TBC1D5 protein exists 89 kDa and 91 kDa isoforms.

实验方案

Product Specific Protocols
WB protocol for TBC1D5 antibody 84990-4-RRDownload protocol
IF protocol for TBC1D5 antibody 84990-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols