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验证数据展示

  • WB analysis using 84914-4-RR

    WB analysis using 84914-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84914-4-RR (TCOF1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of A549 using 84914-4-RR

    WB analysis of A549 using 84914-4-RR

    A549 cells were subjected to SDS PAGE followed by western blot with 84914-4-RR (TCOF1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of MCF-7 using 84914-4-RR

    IF Staining of MCF-7 using 84914-4-RR

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using TCOF1 antibody (84914-4-RR, Clone: 242201E12 ) at dilution of 1:600 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

TCOF1 Recombinant antibody

TCOF1 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 84914-4-RR

产品说明书

CloneNo. 242201E12

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

242201E12, MFD1, treacle

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells, A549 cells, HEK-293T cells
Positive IF/ICC detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy TCOF1 Ready-To-Use IHC Kit. TCOF1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84914-4-RR targets TCOF1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 TCOF1 fusion protein Ag1447 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 Treacher Collins-Franceschetti syndrome 1
别名 242201E12, MFD1, treacle
计算分子量 97 kDa, 144 kDa, 152 kDa
观测分子量220-240 kDa
GenBank蛋白编号BC014559
基因名称 TCOF1
Gene ID (NCBI) 6949
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ13428
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TCOF1 also known as treacle, is a nucleolar protein with a LIS1 homology domain. TCOF1 is involved in ribosomal DNA gene transcription by interacting with upstream binding factor (UBF). Mutations in TCOF1 have been associated with Treacher Collins syndrome, a disorder which includes abnormal craniofacial development(PMID: 31218872)

实验方案

Product Specific Protocols
WB protocol for TCOF1 antibody 84914-4-RRDownload protocol
IF protocol for TCOF1 antibody 84914-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols