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验证数据展示

  • WB analysis using 84885-4-RR

    WB analysis using 84885-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84885-4-RR (Glutamate receptor 3/GluA3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 84885-4-RR

    IHC staining of mouse brain using 84885-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84885-4-RR (Glutamate receptor 3/GluA3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 84885-4-RR

    IHC staining of mouse brain using 84885-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84885-4-RR (Glutamate receptor 3/GluA3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse brain using 84885-4-RR

    IF Staining of mouse brain using 84885-4-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using Glutamate receptor 3/GluA3 antibody (84885-4-RR, Clone: 242392C11 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Glutamate receptor 3/GluA3 Recombinant monoclonal antibody

Glutamate receptor 3/GluA3 Uni-rAb® Recombinant Antibody for WB, IHC, IF-P, ELISA
Cat No. 84885-4-RR

产品说明书

CloneNo. 242392C11

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, ELISA

别名

GRIA3, 242392C11, GluA3, GluR 3, GLUR C

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84885-4-RR targets Glutamate receptor 3/GluA3 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag30898

Product name: Recombinant human GRIA3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 288-425 aa of NM_007325

Sequence: VRLDEREFPEAKNAPLKYTSALTHDAILVIAEAFRYLRRQRVDVSRRGSAGDCLANPAVPWSQGIDIERALKMVQVQGMTGNIQFDTYGRRTNYTIDVYEMKVSGSRKAGYWNEYERFVPFSDQQISNDSASSENRTI

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 glutamate receptor, ionotrophic, AMPA 3
别名 GRIA3, 242392C11, GluA3, GluR 3, GLUR C
计算分子量 101 kDa
观测分子量 101 kDa
GenBank蛋白编号NM_007325
基因名称 GRIA3
Gene ID (NCBI) 2892
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDP42263
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. GRIA3 (Glutamate ionotropic receptor AMPA type subunit 3), also known as Glutamate receptor R3 or A3 (GluR3 or GluA3), is associated with X-linked intellectual disability (ID), dysmorphic features, and non-syndromic epilepsy(PMID: 32977175). GRIA3 also plays a role as a mediator of tumor progression in pancreatic cancer downstream CUX1(PMID: 20689760).

实验方案

Product Specific Protocols
IF protocol for Glutamate receptor 3/GluA3 antibody 84885-4-RRDownload protocol
IHC protocol for Glutamate receptor 3/GluA3 antibody 84885-4-RRDownload protocol
WB protocol for Glutamate receptor 3/GluA3 antibody 84885-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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