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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 84796-3-RR (same clone as 84796-3-PBS)

    WB analysis using 84796-3-RR (same clone as 84796-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84796-3-RR (CD83 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84796-3-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP01570-3

    Sandwich ELISA standard curve of MP01570-3

    Sandwich ELISA standard curve of MP01570-3, Human CD83 Recombinant Matched Antibody Pair - PBS only. 84796-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg2033. 84796-3-PBS was HRP conjugated as the detection antibody. Range: 19.5-1250 pg/mL

  • Sample test of MP01570-3

    Sample test of MP01570-3

    Serum of six individual healthy human donors was measured. The human CD83 concentration of detected samples was determined to be 372.6 pg/mL with a range of 167.8 - 934.1 pg/mL.

  • Cytometric bead array standard curve of MP01570-1

    Cytometric bead array standard curve of MP01570-1

    Cytometric bead array standard curve of MP01570-1, CD83 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84796-1-PBS. Detection antibody: 84796-3-PBS. Standard: Eg2033. Range: 0.313-40 ng/mL.

CD83 Recombinant antibody, PBS Only (Detector)

CD83 Uni-rAbTM Recombinant Antibody for WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 84796-3-PBS

产品说明书

CloneNo. 241855G5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

别名

CD83 molecule, CD83 antigen, BL11, B-cell activation protein, B cell activation protein

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

84796-3-PBS targets CD83 as part of a matched antibody pair:

MP01570-1: 84796-1-PBS capture and 84796-3-PBS detection (validated in Cytometric bead array)

MP01570-3: 84796-4-PBS capture and 84796-3-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 human
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 CD83 molecule
别名 CD83 molecule, CD83 antigen, BL11, B-cell activation protein, B cell activation protein
计算分子量23 kDa
观测分子量40-45 kDa
GenBank蛋白编号NM_004233.4
基因名称 CD83
Gene ID (NCBI) 9308
ENSEMBL Gene IDENSG00000112149
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ01151
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD83 is a member of the immunoglobulin (Ig) superfamily, consisting of an extracellular V-type Ig-like domain, a transmembrane domain, and a cytoplasmic tail. CD83 is one of the most prominent markers for fully matured dendritic cells (DCs) (PMID: 17334966). It is also found on the surface of other activated hematopoietic cells, including lymphocytes, monocytes, macrophages, and neutrophils (PMID: 31231400; 17334966). CD83 regulates the maturation, activation, and homeostasis of numerous immune cells (PMID: 31231400; 32362900). CD83 can also expressed as a soluble form. Soluble CD83 can bind to DCs and inhibit their maturation (PMID: 17334966; 12403928).