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验证数据展示

  • Dot Blot experiment of peptide using 84702-1-RR

    Dot Blot experiment of peptide using 84702-1-RR

    Dot blot analysis was used to confirm the specificity of 84702-1-RR Di-Methyl-Histone H3 (Arg2) antibody. Peptides were spotted onto NC and probed with antibody at 1 µg/ml. The amount of peptide (ug/mL) spotted is indicated next to each row.

  • ChIP experiment of HeLa using 84702-1-RR

    ChIP experiment of HeLa using 84702-1-RR

    Chromatin was prepared from HeLa cells. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 15 µg of cross-linked chromatin, 5 µg of Di-Methyl-Histone H3 (Arg2) (84702-1-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR.

  • IHC staining of human placenta using 84702-1-RR

    IHC staining of human placenta using 84702-1-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84702-1-RR (Di-Methyl-Histone H3 (Arg2) antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Phospho-Histone H3 (Ser10) antibody included
  • IHC staining of rat liver using 84702-1-RR

    IHC staining of rat liver using 84702-1-RR

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 84702-1-RR (Di-Methyl-Histone H3 (Arg2) antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Phospho-Histone H3 (Ser10) antibody included
  • IHC staining of mouse liver using 84702-1-RR

    IHC staining of mouse liver using 84702-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84702-1-RR (Di-Methyl-Histone H3 (Arg2) antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Phospho-Histone H3 (Ser10) antibody included
  • IF Staining of HeLa using 84702-1-RR

    IF Staining of HeLa using 84702-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HIST1H3A antibody (84702-1-RR, Clone: 241896D7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • IF Staining of NIH/3T3 using 84702-1-RR

    IF Staining of NIH/3T3 using 84702-1-RR

    Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using Di-Methyl-Histone H3 (Arg2) antibody (84702-1-RR, Clone: 241896D7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • IF Staining of C6 using 84702-1-RR

    IF Staining of C6 using 84702-1-RR

    Immunofluorescent analysis of (4% PFA) fixed C6 cells using Di-Methyl-Histone H3 (Arg2) antibody (84702-1-RR, Clone: 241896D7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • FC experiment of HeLa using 84702-1-RR

    FC experiment of HeLa using 84702-1-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Hist1h3a Recombinant Antibody (84702-1-RR, Clone:241896D7) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

Di-Methyl-Histone H3 (Arg2) Recombinant monoclonal antibody

Di-Methyl-Histone H3 (Arg2) Uni-rAb® Recombinant Antibody for IHC, IF/ICC, FC (Intra), Dot Blot, ELISA, ChIP-qPCR
Cat No. 84702-1-RR

产品说明书

CloneNo. 241896D7

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IHC, IF/ICC, FC (Intra), Dot Blot, ELISA, ChIP-qPCR

别名

Di Methyl Histone H3, Di-Methyl Histone H3 (Arg2), Di-Methyl Histone H3 Arg2, Di-Methyl Histone-H3 Arg2, Di-Methyl-Histone H3

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive IHC detected inrat liver tissue, human placenta tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, NIH/3T3 cells, C6 cells
Positive FC (Intra) detected inHeLa cells
Positive Dot Blot detected inpeptide
Positive ChIP-qPCR detected inHeLa cells

This antibody specifically recognize symmetric dimethyl-arginine (SDMA, Rme2s).

Planning an IHC experiment? We recommend our IHCeasy Phospho-Histone H3 (Ser10) Ready-To-Use IHC Kit. Phospho-Histone H3 (Ser10) primary antibody included.

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
DOT BLOTDOT BLOT : 1:10-1:100
CHIP-QPCRCHIP-QPCR : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84702-1-RR targets Di-Methyl-Histone H3 (Arg2) in IHC, IF/ICC, FC (Intra), Dot Blot, ELISA, ChIP-qPCR applications and shows reactivity with human, mouse, rat samples.

经测试应用 IHC, IF/ICC, FC (Intra), Dot Blot, ELISA, ChIP-qPCR Application Description
经测试反应性 human, mouse, rat
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 histone cluster 1, H3a
别名 Di Methyl Histone H3, Di-Methyl Histone H3 (Arg2), Di-Methyl Histone H3 Arg2, Di-Methyl Histone-H3 Arg2, Di-Methyl-Histone H3
GenBank蛋白编号BC066245
基因名称 HIST1H3A
Gene ID (NCBI) 8350
RRIDAB_3672118
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDP68431
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Arginine dimethylation is an important post-translational modification of proteins. Based on the positions of the two methyl groups on the guanidino group of arginine, it can be divided into symmetric dimethylation (SDMA) and asymmetric dimethylation (ADMA). Symmetric dimethylation is crucial for embryonic development and cell differentiation and is often associated with transcriptional repression. This antibody specifically recognizes symmetric dimethyl-arginine (SDMA, Rme2s).

实验方案

Product Specific Protocols
IF protocol for Di-Methyl-Histone H3 (Arg2) antibody 84702-1-RRDownload protocol
IHC protocol for Di-Methyl-Histone H3 (Arg2) antibody 84702-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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