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验证数据展示

  • WB analysis of Recombinant protein using 84687-2-RR (same clone as 84687-2-PBS)

    WB analysis of Recombinant protein using 84687-2-RR (same clone as 84687-2-PBS)

    Recombinant protein VEGF121 (Eg1263) was subjected to SDS PAGE followed by western blot with 84687-2-RR (VEGFA antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84687-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01495-1

    Cytometric bead array standard curve of MP01495-1

    Cytometric bead array standard curve of MP01495-1, VEGF Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84687-1-PBS. Detection antibody: 84687-2-PBS. Standard: Eg1263. Range: 0.625-80 ng/mL.

  • Affinity and Kinetic Characterization of 84687-2-PBS

    Affinity and Kinetic Characterization of 84687-2-PBS

    Biolayer interferometry (BLl) kinetic assay of 84687-2-PBS against Human VEGF121 was performed. The affinity constant is 0.882 nM.

  • Affinity and Kinetic Characterization of 84687-2-RR

    Affinity and Kinetic Characterization of 84687-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84687-2-RR against Human VEGFA were performed. The affinity constant is 92.0 pM.

VEGFA Recombinant antibody, PBS Only (Detector)

VEGFA Uni-rAbTM Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA
Cat No. 84687-2-PBS

产品说明书

CloneNo. 242072G9

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, Cytometric bead array, Indirect ELISA

别名

VEGF, Vascular endothelial growth factor A, long form, vascular endothelial growth factor A, Vascular Endothelial Growth Factor, N-VEGF

缓冲液配方:  PBS Only
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy VEGFA/VEGF Ready-To-Use IHC Kit. VEGFA/VEGF primary antibody included.

产品信息

84687-2-PBS targets VEGFA as part of a matched antibody pair:

MP01495-1: 84687-1-PBS capture and 84687-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 vascular endothelial growth factor A
别名 VEGF, Vascular endothelial growth factor A, long form, vascular endothelial growth factor A, Vascular Endothelial Growth Factor, N-VEGF
GenBank蛋白编号NM_001171628.1
基因名称 VEGFA
Gene ID (NCBI) 7422
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP15692-9
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

VEGFA, also named VEGF or VPF, belongs to the PDGF/VEGF growth factor family. It is a growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. VEGFA induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. It binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. Defects in VEGFA are associated with microvascular complications of diabetes type 1 (MVCD1). VEGFA has 17 isoforms with MW from 16 to 45 kDa. Some isoforms have homodimer forms (e.g.; VEGFA189 38 kDa or VEFGA110 34 kDa). VEGF-A exists in at least seven homodimeric isoforms. The monomers consist of 121, 145, 148, 165, 183, 189, or 206 amino acids (PMID:15602010 ).