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验证数据展示

  • IHC staining of mouse skeletal muscle using 84425-5-RR

    IHC staining of mouse skeletal muscle using 84425-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 84425-5-RR (PERM1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse skeletal muscle using 84425-5-RR

    IHC staining of mouse skeletal muscle using 84425-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 84425-5-RR (PERM1 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of H9C2 using 84425-5-RR

    IF Staining of H9C2 using 84425-5-RR

    Immunofluorescent analysis of (4% PFA) fixed H9C2 cells using PERM1 antibody (84425-5-RR, Clone: 241613B6 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 84425-5-RR

    Affinity and Kinetic Characterization of 84425-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84425-5-RR against Human PERM1 were performed. The affinity constant is below 1 pM.

PERM1 Recombinant antibody

PERM1 Uni-rAbTM Recombinant Antibody for IHC, IF/ICC, ELISA
Cat No. 84425-5-RR

产品说明书

CloneNo. 241613B6

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

IHC, IF/ICC, ELISA

别名

C1orf170, PERM, 241613B6

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IHC detected inmouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inH9C2 cells

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84425-5-RR targets PERM1 in IHC, IF/ICC, ELISA applications and shows reactivity with human, rat samples.

经测试应用 IHC, IF/ICC, ELISA Application Description
经测试反应性 human, rat
免疫原 PERM1 fusion protein Ag23833 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 chromosome 1 open reading frame 170
别名 C1orf170, PERM, 241613B6
GenBank蛋白编号BC006300
基因名称 C1orf170
Gene ID (NCBI) 84808
RRIDAB_3671952
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ5SV97
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PERM1 (PGC-1/ERR-induced regulator in muscle 1) is a muscle-specific protein induced by PGC-1α and ERRα. PERM1 protein expression is mainly restricted to heart and skeletal muscle, indicating PERM1 functions as a tissue-specific regulator of mitochondrial biogenesis in response to exercise (PMID: 33549681, 36028747). Ablation of Perm1 in mice results in reduced protein expression of lipin-1 accompanied by accumulation of specific phospholipid species (PMID: 33549681). There are two major isoforms of Perm1 protein, the short isoform is about 85 kDa, and the long isoform is 105 kDa.

实验方案

Product Specific Protocols
IHC protocol for PERM1 antibody 84425-5-RRDownload protocol
IF protocol for PERM1 antibody 84425-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols