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验证数据展示

  • IHC staining of mouse liver using 84316-7-RR

    IHC staining of mouse liver using 84316-7-RR

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84316-7-RR (HNF4A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 84316-7-RR

    IF Staining of HepG2 using 84316-7-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HNF4A antibody (84316-7-RR, Clone: 241593A2 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 84316-7-RR

    Affinity and Kinetic Characterization of 84316-7-RR

    Biolayer interferometry (BLl) kinetic assays of 84316-7-RR against Human HNF4A were performed. The affinity constant is 0.275 nM.

HNF4A Recombinant antibody

HNF4A Uni-rAbTM Recombinant Antibody for IHC, IF/ICC, ELISA
Cat No. 84316-7-RR

产品说明书

CloneNo. 241593A2

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, IF/ICC, ELISA

别名

241593A2, Hepatocyte nuclear factor 4-alpha, HNF 4 alpha, HNF4, HNF4a7

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84316-7-RR targets HNF4A in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 HNF4A fusion protein Ag24647 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 hepatocyte nuclear factor 4, alpha
别名 241593A2, Hepatocyte nuclear factor 4-alpha, HNF 4 alpha, HNF4, HNF4a7
计算分子量 53 kDa
GenBank蛋白编号BC137539
基因名称 HNF4A
Gene ID (NCBI) 3172
RRIDAB_3671857
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP41235
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HNF4A (Hepatocyte nuclear factor 4 α) is highly conserved between humans and mice. Using conditional knockouts, epithelial HNF4A is indispensable for protecting against acute chemical-induced colitis and spontaneous colonic inflammation in aged animals (PMID:35792863). Moreover, reduced expression of HNF4A has been reported in liver fibrosis and viral vector-mediated HNF4A restoration has previously been demonstrated to reverse features of chronic liver injury. Therefore, HNF4A has been suggested as a therapeutic target for the treatment of liver fibrosis (PMID:34453962).

实验方案

Product Specific Protocols
IHC protocol for HNF4A antibody 84316-7-RRDownload protocol
IF protocol for HNF4A antibody 84316-7-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols