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验证数据展示

  • WB analysis using 84239-4-RR (same clone as 84239-4-PBS)

    WB analysis using 84239-4-RR (same clone as 84239-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84239-4-RR (APOBEC3A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84239-4-PBS in a different storage buffer formulation.

  • IF Staining of THP-1 using 84239-4-RR (same clone as 84239-4-PBS)

    IF Staining of THP-1 using 84239-4-RR (same clone as 84239-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using APOBEC3A antibody (84239-4-RR, Clone: 241529G4 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84239-4-PBS in a different storage buffer formulation.

  • FC experiment of A431 using 84239-4-RR (same clone as 84239-4-PBS)

    FC experiment of A431 using 84239-4-RR (same clone as 84239-4-PBS)

    1x10^6 A431 cells were intracellularly stained with 0.25 ug Apobec3a Recombinant Antibody (84239-4-RR, Clone:241529G4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 84239-4-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01122-2

    Cytometric bead array standard curve of MP01122-2

    Cytometric bead array standard curve of MP01122-2, APOBEC3A Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84239-1-PBS. Detection antibody: 84239-4-PBS. Standard: Ag18845. Range: 0.625-80 ng/mL.

  • Affinity and Kinetic Characterization of 84239-4-PBS

    Affinity and Kinetic Characterization of 84239-4-PBS

    Biolayer interferometry (BLl) kinetic assay of 84239-4-PBS against Human APOBEC3A was performed. The affinity constant is 0.471 nM.

  • Affinity and Kinetic Characterization of 84239-4-RR

    Affinity and Kinetic Characterization of 84239-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84239-4-RR against Human APOBEC3A were performed. The affinity constant is 0.328 nM.

APOBEC3A Recombinant antibody, PBS Only (Detector)

APOBEC3A Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA
Cat No. 84239-4-PBS

产品说明书

CloneNo. 241529G4

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

别名

241529G4, A3A, ARP3, DNA dC->dU-editing enzyme APOBEC-3A, EC:3.5.4.38

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

84239-4-PBS targets APOBEC3A as part of a matched antibody pair:

MP01122-2: 84239-1-PBS capture and 84239-4-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, rat
免疫原 APOBEC3A fusion protein Ag18845 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3A
别名 241529G4, A3A, ARP3, DNA dC->dU-editing enzyme APOBEC-3A, EC:3.5.4.38
计算分子量 199 aa, 23 kDa
观测分子量28 kDa
GenBank蛋白编号BC126416
基因名称 APOBEC3A
Gene ID (NCBI) 200315
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP31941
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

APOBEC3A, also known as A3A, belongs to the cytidine and deoxycytidylate deaminase family. APOBEC3A plays a role in immunity, by restricting transmission of foreign DNA such as viruses. One mechanism of foreign DNA restriction is deamination of foreign double-stranded DNA cytidines to uridines, which leads to DNA degradation. However, other mechanisms are also thought to be involved, as anti-viral effect is not dependent on deaminase activity. APOBEC3A is expressed in several cell types, including keratinocytes and myeloid cells (PMID: 28825669). APOBEC3A has 2 isoforms with the molecular mass of 22 and 23 kDa.