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验证数据展示

  • WB analysis using 84239-4-RR

    WB analysis using 84239-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84239-4-RR (APOBEC3A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of THP-1 using 84239-4-RR

    IF Staining of THP-1 using 84239-4-RR

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using APOBEC3A antibody (84239-4-RR, Clone: 241529G4 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • FC experiment of A431 using 84239-4-RR

    FC experiment of A431 using 84239-4-RR

    1x10^6 A431 cells were intracellularly stained with 0.25 ug Apobec3a Recombinant Antibody (84239-4-RR, Clone:241529G4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Affinity and Kinetic Characterization of 84239-4-RR

    Affinity and Kinetic Characterization of 84239-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84239-4-RR against Human APOBEC3A were performed. The affinity constant is 0.328 nM.

APOBEC3A Recombinant monoclonal antibody

APOBEC3A Uni-rAb® Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 84239-4-RR

产品说明书

CloneNo. 241529G4

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, IF/ICC, FC (Intra), ELISA

别名

241529G4, A3A, ARP3, DNA dC->dU-editing enzyme APOBEC-3A, EC:3.5.4.38

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inrat colon tissue, HeLa cells, HaCaT cells
Positive IF/ICC detected inTHP-1 cells
Positive FC (Intra) detected inA431 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84239-4-RR targets APOBEC3A in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human, rat
免疫原

CatNo: Ag18845

Product name: Recombinant human APOBEC3A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-61 aa of BC126416

Sequence: MEASPASGPRHLMDPHIFTSNFNNGIGRHKTYLCYEVERLDNGTSVKMDQHRGFLHNQAKN

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3A
别名 241529G4, A3A, ARP3, DNA dC->dU-editing enzyme APOBEC-3A, EC:3.5.4.38
计算分子量 199 aa, 23 kDa
观测分子量28 kDa
GenBank蛋白编号BC126416
基因名称 APOBEC3A
Gene ID (NCBI) 200315
RRIDAB_3671792
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP31941
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

APOBEC3A, also known as A3A, belongs to the cytidine and deoxycytidylate deaminase family. APOBEC3A plays a role in immunity, by restricting transmission of foreign DNA such as viruses. One mechanism of foreign DNA restriction is deamination of foreign double-stranded DNA cytidines to uridines, which leads to DNA degradation. However, other mechanisms are also thought to be involved, as anti-viral effect is not dependent on deaminase activity. APOBEC3A is expressed in several cell types, including keratinocytes and myeloid cells (PMID: 28825669). APOBEC3A has 2 isoforms with the molecular mass of 22 and 23 kDa.

实验方案

Product Specific Protocols
IF protocol for APOBEC3A antibody 84239-4-RRDownload protocol
WB protocol for APOBEC3A antibody 84239-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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