Various lysates were subjected to SDS PAGE followed by western blot with 84131-2-RR (EIF3F antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84131-2-PBS in a different storage buffer formulation.

The mean EIF3F concentration was determined to be 225.37 ng/mL in Jurkat cell extract based on a 1.60 mg/mL extract load.

Sandwich ELISA standard curve of MP01038-4, Human EIF3F Recombinant Matched Antibody Pair - PBS only. 84131-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag34849. 84131-2-PBS was HRP conjugated as the detection antibody. Range: 0.469-30 ng/mL.

Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using EIF3F antibody (84131-2-RR, Clone: 240894D2 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84131-2-PBS in a different storage buffer formulation.

1x10^6 HeLa cells were intracellularly stained with 0.25 ug EIF3F Recombinant antibody (84131-2-RR, Clone:240894D2) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 84131-2-PBS in a different storage buffer formulation.

Biolayer interferometry (BLl) kinetic assays of 84131-2-RR against Human EIF3F were performed. The affinity constant is 1.78 nM.