验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, RAW 264.7 cells, C6 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
84062-3-RR targets LRRK2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag33713 Product name: Recombinant human LRRK2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 2372-2527 aa of NM_198578 Sequence: VVEVWDKKTEKLCGLIDCVHFLREVMVKENKESKHKMSYSGRVKTLCLQKNTALWIGTGGGHILLLDLSTRRLIRVIYNFCNSVRVMMTAQLGSLKNVMLVLGYNRKNTEGTQKQKEIQSCLTVWDINLPHEVQNLEKHIEVRKELAEKMRRTSVE* 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | leucine-rich repeat kinase 2 |
| 别名 | AURA17, Dardarin, EC:2.7.11.1, leucine rich repeat kinase 2, Leucine-rich repeat serine/threonine-protein kinase 2 |
| 计算分子量 | 286 kDa |
| 观测分子量 | 286 kDa |
| GenBank蛋白编号 | NM_198578 |
| 基因名称 | LRRK2 |
| Gene ID (NCBI) | 120892 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purfication |
| UNIPROT ID | Q5S007 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Leucine-rich repeat kinase 2 (LRRK2) in humans is encoded by the PARK8/LRRK2 gene. Genetic variations within the LRRK2 gene are linked to a number of diseases, including Parkinson's disease (PD), Crohn's disease and Hansen's disease. The most frequent pathogenic mutations reside in the ROC-COR GTPase (R1441G/C/H, Y1669C) and kinase domains (G2019S, I2020T), indicating important roles for both enzymatic domains in the pathogenicity of LRRK2-driven PD. The G2019S mutation in the Leucine-rich repeat kinase-2 (LRRK2) protein is the most common pathogenic mutation, accounting for 1-6% of sporadic and 3-19% of familial PD. (PMID: 34991886,PMID: 30872638)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for LRRK2 antibody 84062-3-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
