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验证数据展示

  • WB analysis using 84034-4-RR

    WB analysis using 84034-4-RR

    A549 cells were subjected to SDS PAGE followed by western blot with 84034-4-RR (L1CAM antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 84034-4-RR

    WB analysis of HeLa using 84034-4-RR

    WB result of L1CAM/CD171 antibody (84034-4-RR; 1:200000; incubated at room temperature for 1.5 hours) with sh-Control and sh-L1CAM/CD171 transfected HeLa cells.

  • Affinity and Kinetic Characterization of 84034-4-RR

    Affinity and Kinetic Characterization of 84034-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84034-4-RR against Human L1CAM were performed. The affinity constant is 0.255 nM.

  • Featured Product
  • KD/KO验证

L1CAM/CD171 Recombinant monoclonal antibody

L1CAM/CD171 Uni-rAb® Recombinant Antibody for WB, ELISA
Cat No. 84034-4-RR

产品说明书

CloneNo. 240978F1

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

L1CAM, 240978F1, CAML1, CD171, HSAS

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA549 cells, HeLa cells
Planning an IHC experiment? We recommend our IHCeasy L1CAM Ready-To-Use IHC Kit. L1CAM primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84034-4-RR targets L1CAM/CD171 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Eg0209

Product name: Recombinant Human L1CAM protein (Myc Tag, His Tag)

Source: mammalian cells-derived, pHZ-KIsec

Tag: Myc & 6*His

Domain: 20-1120 aa of BC126229

Sequence: IQIPEEYEGHHVMEPPVITEQSPRRLVVFPTDDISLKCEASGKPEVQFRWTRDGVHFKPKEELGVTVYQSPHSGSFTITGNNSNFAQRFQGIYRCFASNKLGTAMSHEIRLMAEGAPKWPKETVKPVEVEEGESVVLPCNPPPSAEPLRIYWMNSKILHIKQDERVTMGQNGNLYFANVLTSDNHSDYICHAHFPGTRTIIQKEPIDLRVKATNSMIDRKPRLLFPTNSSSHLVALQGQPLVLECIAEGFPTPTIKWLRPSGPMPADRVTYQNHNKTLQLLKVGEEDDGEYRCLAENSLGSARHAYYVTVEAAPYWLHKPQSHLYGPGETARLDCQVQGRPQPEVTWRINGIPVEELAKDQKYRIQRGALILSNVQPSDTMVTQCEARNRHGLLLANAYIYVVQLPAKILTADNQTYMAVQGSTAYLLCKAFGAPVPSVQWLDEDGTTVLQDERFFPYANGTLGIRDLQANDTGRYFCLAANDQNNVTIMANLKVKDATQITQGPRSTIEKKGSRVTFTCQASFDPSLQPSITWRGDGRDLQELGDSDKYFIEDGRLVIHSLDYSDQGNYSCVASTELDVVESRAQLLVVGSPGPVPRLVLSDLHLLTQSQVRVSWSPAEDHNAPIEKYDIEFEDKEMAPEKWYSLGKVPGNQTSTTLKLSPYVHYTFRVTAINKYGPGEPSPVSETVVTPEAAPEKNPVDVKGEGNETTNMVITWKPLRWMDWNAPQVQYRVQWRPQGTRGPWQEQIVSDPFLVVSNTSTFVPYEIKVQAVNSQGKGPEPQVTIGYSGEDYPQAIPELEGIEILNSSAVLVKWRPVDLAQVKGHLRGYNVTYWREGSQRKHSKRHIHKDHVVVPANTTSVILSGLRPYSSYHLEVQAFNGRGSGPASEFTFSTPEGVPGHPEALHLECQSNTSLLLRWQPPLSHNGVLTGYVLSYHPLDEGGKGQLSFNLRDPELRTHNLTDLSPHLRYRFQLQATTKEGPGEAIVREGGTMALSGISDFGNISATAGENYSVVSWVPKEGQCNFRFHILFKALGEEKGGASLSPQYVSYNQSSYTQWDLQPDTDYEIHLFKERMFRHQMAVKTNGTGRVRLPPAGFATE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 L1 cell adhesion molecule
别名 L1CAM, 240978F1, CAML1, CD171, HSAS
计算分子量 1257 aa, 140 kDa
观测分子量200-250 kDa
GenBank蛋白编号BC126229
基因名称 L1CAM
Gene ID (NCBI) 3897
RRIDAB_3671602
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDP32004
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

L1CAM, also known as NCAM-L1 or CD171, is a cell adhesion molecule of the immunoglobulin superfamily. It is a 200-220 kDa transmembrane glycoprotein composed of six Ig-like domains and five fibronectin type III repeats followed by a transmembrane region and a highly conserved cytoplasmic tail (PMID: 3412448; 22796939). L1CAM is primarily expressed in the nervous system and is involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, cerebellar granule cell migration, neurite outgrowth on Schwann cells and interactions among epithelial cells of intestinal crypts (PMID: 3412448; 10767310). L1CAM is overexpressed in many human cancers and is often associated with bad prognosis (PMID: 27267927; 26111503).

实验方案

Product Specific Protocols
WB protocol for L1CAM/CD171 antibody 84034-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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