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验证数据展示

  • WB analysis using 83992-1-RR

    WB analysis using 83992-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83992-1-RR (CCDC22 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 83992-1-RR

    WB analysis of HepG2 using 83992-1-RR

    WB result of CCDC22 antibody (83992-1-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CCDC22 transfected HepG2 cells.

  • Affinity and Kinetic Characterization of 83992-1-RR

    Affinity and Kinetic Characterization of 83992-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83992-1-RR against Human CCDC22 were performed. The affinity constant is 2.71 nM.

  • Featured Product
  • KD/KO验证

CCDC22 Recombinant monoclonal antibody

CCDC22 Uni-rAb® Recombinant Antibody for WB, ELISA
Cat No. 83992-1-RR

产品说明书

CloneNo. 241146A5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

JM1, CXorf37, Coiled-coil domain-containing protein 22, Coiled coil domain containing protein 22, coiled coil domain containing 22

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, HEK-293 cells, Jurkat cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83992-1-RR targets CCDC22 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag9980

Product name: Recombinant human CCDC22 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 283-628 aa of BC000972

Sequence: AKTGAPKGSRFTHSEKFTFHLEPQAQATQVSDVPATSRRPEQVTWAAQEQELESLREQLEGVNRSIEEVEADMKTLGVSFVQAESECRHSKLSTAEREQALRLKSRAVELLPDGTANLAKLQLVVENSAQRVIHLAGQWEKHRVPLLAEYRHLRKLQDCRELESSRRLAEIQELHQSVRAAAEEARRKEEVYKQLMSELETLPRDVSRLAYTQRILEIVGNIRKQKEEITKILSDTKELQKEINSLSGKLDRTFAVTDELVFKDAKKDDAVRKAYKYLAALHENCSQLIQTIEDTGTIMREVRDLEEQIETELGKKTLSNLEKIREDYRALRQENAGLLGRVREA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 coiled-coil domain containing 22
别名 JM1, CXorf37, Coiled-coil domain-containing protein 22, Coiled coil domain containing protein 22, coiled coil domain containing 22
计算分子量 71 kDa
观测分子量71 kDa
GenBank蛋白编号BC000972
基因名称 CCDC22
Gene ID (NCBI) 28952
RRIDAB_3671567
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO60826
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CCDC22 is a ubiquitously expressed coiled-coil domain protein. CCDC22 functions in the regulation of NF-kB (nuclear factor kappa-light-chain-enhancer of activated B cells) by interacting with COMMD (copper metabolism Murr1 domain-containing) proteins. ]

实验方案

Product Specific Protocols
WB protocol for CCDC22 antibody 83992-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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